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本文引用的文献

1
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Am J Physiol. 1980 Jun;238(6):F461-9. doi: 10.1152/ajprenal.1980.238.6.F461.
2
Increased Na+-H+ exchange in brush border vesicles from dogs with renal failure.肾衰竭犬刷状缘小泡中钠氢交换增加。
Am J Physiol. 1982 Sep;243(3):F293-9. doi: 10.1152/ajprenal.1982.243.3.F293.
3
Determination of the coupling ratio for Na+ -H+ exchange in renal microvillus membrane vesicles.肾微绒毛膜囊泡中Na⁺-H⁺交换偶联比率的测定。
Biochim Biophys Acta. 1982 Jul 14;689(1):161-4. doi: 10.1016/0005-2736(82)90200-0.
4
Sodium and chloride transport across rabbit ileal brush border. I. Evidence for Na-H exchange.钠和氯跨兔回肠刷状缘的转运。I. 钠-氢交换的证据。
Am J Physiol. 1983 Oct;245(4):G504-10. doi: 10.1152/ajpgi.1983.245.4.G504.
5
The role of the Na+/H+ exchange system in cardiac cells in relation to the control of the internal Na+ concentration. A molecular basis for the antagonistic effect of ouabain and amiloride on the heart.钠/氢交换系统在心肌细胞中对细胞内钠浓度控制的作用。哇巴因和阿米洛利对心脏拮抗作用的分子基础。
J Biol Chem. 1984 Jul 25;259(14):8880-5.
6
The plasma membrane sodium-hydrogen exchanger and its role in physiological and pathophysiological processes.质膜钠氢交换体及其在生理和病理生理过程中的作用。
Circ Res. 1985 Jun;56(6):773-88. doi: 10.1161/01.res.56.6.773.
7
Regulation of intracellular pH in eukaryotic cells.真核细胞内pH值的调节
Biochem J. 1988 Feb 15;250(1):1-8. doi: 10.1042/bj2500001.
8
Sodium influx in human leucocytes: a novel technique for assessment of sodium-proton antiport activity.人白细胞中的钠内流：一种评估钠-质子反向转运活性的新技术。
Clin Sci (Lond). 1988 Aug;75(2):179-84. doi: 10.1042/cs0750179.
9
Leucocyte sodium pump activity after meals or insulin in normal and obese subjects: cause for increased energetic efficiency in obesity?正常人和肥胖者进食或注射胰岛素后的白细胞钠泵活性：肥胖者能量效率提高的原因？
Br Med J (Clin Res Ed). 1987 Nov 28;295(6610):1369-73. doi: 10.1136/bmj.295.6610.1369.
10
Sodium/proton antiport in brush-border-membrane vesicles isolated from rat small intestine and kidney.从大鼠小肠和肾脏分离的刷状缘膜囊泡中的钠/质子反向转运
Biochem J. 1976 Mar 15;154(3):597-604.

随着pH值降低，人白细胞钠氢反向转运体化学计量发生改变。

Altered stoichiometry of the human leucocyte Na+/H+ antiport with decreasing pH.

作者信息

Ng L L, Bomford J

机构信息

Department of Clinical Pharmacology, Radcliffe Infirmary, Oxford, U.K.

出版信息

Biochem J. 1989 Apr 1;259(1):311-4. doi: 10.1042/bj2590311.

DOI:10.1042/bj2590311
PMID:2541685
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1138508/
Abstract

The Na+/H+ antiport is an important regulator of cellular volume, pH and Na+ concentration in mammalian cells. The stoichiometry of this antiporter has previously been shown to be a 1:1 exchange of internal H+ for external Na+. We have investigated this stoichiometry in human leucocytes by using a novel intracellular pH-clamping technique and measuring 22Na+ influx and H+ efflux in the same cells. As internal pH was lowered, the stoichiometry of H+/Na+ exchange rose to a mean +/- S.D. of 2.23 +/- 0.69. This mechanism allows a higher H+ efflux in the face of intracellular acid stress without causing excessive intracellular Na+ overload.

摘要

Na+/H+逆向转运蛋白是哺乳动物细胞中细胞体积、pH值和Na+浓度的重要调节因子。此前已证明该逆向转运蛋白的化学计量为细胞内H+与细胞外Na+以1:1的比例进行交换。我们通过使用一种新型的细胞内pH钳制技术，并测量同一细胞中的22Na+内流和H+外流，对人类白细胞中的这种化学计量进行了研究。随着细胞内pH值降低，H+/Na+交换的化学计量上升至平均值±标准差为2.23±0.69。这种机制使得在细胞内酸应激情况下能够有更高的H+外流，而不会导致细胞内Na+过度超载。