随着pH值降低，人白细胞钠氢反向转运体化学计量发生改变。
Altered stoichiometry of the human leucocyte Na+/H+ antiport with decreasing pH.
作者信息
Ng L L, Bomford J
机构信息
Department of Clinical Pharmacology, Radcliffe Infirmary, Oxford, U.K.
出版信息
Biochem J. 1989 Apr 1;259(1):311-4. doi: 10.1042/bj2590311.
Abstract
The Na+/H+ antiport is an important regulator of cellular volume, pH and Na+ concentration in mammalian cells. The stoichiometry of this antiporter has previously been shown to be a 1:1 exchange of internal H+ for external Na+. We have investigated this stoichiometry in human leucocytes by using a novel intracellular pH-clamping technique and measuring 22Na+ influx and H+ efflux in the same cells. As internal pH was lowered, the stoichiometry of H+/Na+ exchange rose to a mean +/- S.D. of 2.23 +/- 0.69. This mechanism allows a higher H+ efflux in the face of intracellular acid stress without causing excessive intracellular Na+ overload.
摘要
Na+/H+逆向转运蛋白是哺乳动物细胞中细胞体积、pH值和Na+浓度的重要调节因子。此前已证明该逆向转运蛋白的化学计量为细胞内H+与细胞外Na+以1:1的比例进行交换。我们通过使用一种新型的细胞内pH钳制技术，并测量同一细胞中的22Na+内流和H+外流，对人类白细胞中的这种化学计量进行了研究。随着细胞内pH值降低，H+/Na+交换的化学计量上升至平均值±标准差为2.23±0.69。这种机制使得在细胞内酸应激情况下能够有更高的H+外流，而不会导致细胞内Na+过度超载。
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