Bardy B, Morinet F, Pérol Y
Department of Virology, University of Helsinki, Finland.
Intervirology. 1989;30(1):36-43. doi: 10.1159/000150074.
The occurrence of Epstein-Barr virus (EBV) specific DNase antibodies was measured in the sera of 400 patients. Three hundred sera were positive (greater than or equal to 20) for early antigen diffuse component (EA-D) antibodies, as measured by indirect immunofluorescence (IF). One hundred sera were EA-D antibody-negative but had IF IgG titers (greater than 40) to the viral capsid antigen (VCA). Twenty-nine of the anti-EA-D-positive sera, and 14 of the anti-EA-D-negative sera showed specific inhibition of EBV DNase. The mean age of positive male patients (39 years) for DNase antibodies was significantly higher (p less than 0.05) than the mean age of positive females (27 years). Thirteen of the 40 patients (29.5%) with acquired immunodeficiency syndrome (AIDS) had EBV DNase-neutralizing antibodies. No correlation between the level of anti-DNase antibodies and the antibody titer to EA-D or VCA was observed. These results support the notion that the EBV DNase antibody assay measures an independent index reflecting factual EBV disease.
对400例患者的血清进行了爱泼斯坦-巴尔病毒（EBV）特异性脱氧核糖核酸酶抗体检测。通过间接免疫荧光法（IF）检测发现，300份血清的早期抗原弥漫成分（EA-D）抗体呈阳性（大于或等于20）。100份血清EA-D抗体阴性，但对病毒衣壳抗原（VCA）的IF IgG滴度大于40。29份抗EA-D阳性血清和14份抗EA-D阴性血清显示出对EBV脱氧核糖核酸酶的特异性抑制。脱氧核糖核酸酶抗体阳性男性患者的平均年龄（39岁）显著高于阳性女性患者的平均年龄（27岁）（p<0.05）。40例获得性免疫缺陷综合征（AIDS）患者中有13例（29.5%）具有EBV脱氧核糖核酸酶中和抗体。未观察到抗脱氧核糖核酸酶抗体水平与EA-D或VCA抗体滴度之间存在相关性。这些结果支持了EBV脱氧核糖核酸酶抗体检测可测量反映实际EBV疾病的独立指标这一观点。
