环磷酸腺苷依赖性蛋白激酶在酿酒酵母中葡萄糖和半乳糖转运体分解代谢失活中的作用。
Role of cyclic-AMP-dependent protein kinase in catabolite inactivation of the glucose and galactose transporters in Saccharomyces cerevisiae.
作者信息
Ramos J, Cirillo V P
机构信息
Department of Biochemistry, State University of New York, Stony Brook 11794.
出版信息
J Bacteriol. 1989 Jun;171(6):3545-8. doi: 10.1128/jb.171.6.3545-3548.1989.
Abstract
The derepressed high-affinity glucose transport system and the induced galactose transport system are catabolite inactivated when cells with these transport systems are incubated with glucose. The role of the cyclic AMP cascade in the catabolite inactivation of these transport systems was shown by using mutants affected in the activity of cyclic-AMP-dependent protein kinase (cAPK). In tpk1(w) mutants with reduced cAPK activity, the sugar transport systems were expressed but were not catabolite inactivated. In bcy1 mutants with unbridled cAPK activity resulting from a defective regulatory subunit, the transport systems were absent or present at low levels.
摘要
当具有这些转运系统的细胞与葡萄糖一起孵育时，去阻遏的高亲和力葡萄糖转运系统和诱导的半乳糖转运系统会被分解代谢物失活。通过使用在环腺苷酸依赖性蛋白激酶（cAPK）活性方面有缺陷的突变体，证明了环腺苷酸级联反应在这些转运系统的分解代谢物失活中的作用。在cAPK活性降低的tpk1(w)突变体中，糖转运系统得以表达，但不会被分解代谢物失活。在由于调节亚基缺陷导致cAPK活性不受控制的bcy1突变体中，转运系统不存在或水平很低。
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