Berg G, Sanjaghsaz H, Wangwongwatana S
Department of Civil and Environmental Engineering, University of Cincinnati, Ohio 45221.
J Virol Methods. 1989 Feb;23(2):179-86. doi: 10.1016/0166-0934(89)90131-6.
Poliovirus 1 was inactivated by free chlorine at pH 9.0 three times more rapidly in boric acid (0.05 M)-NaOH buffer than in purified (carbon-filtered, deionized) water. Thus, at a given concentration of free chlorine, it took three times longer to inactivate the same fraction of the poliovirus in purified water than in the boric acid-NaOH buffer. Conversely, in a given period of time, three times more chlorine was required to inactivate a given percentage of the virus in purified water than in the boric acid-NaOH buffer. Buffers are almost always used to control pH in disinfection studies with free chlorine and with other chlorine compounds also. The use of buffers for pH control in such disinfection studies may distort the resulting data and, at least for waters that contain little salt, may cause serious overestimation of the rates at which viruses are inactivated.
在pH 9.0的条件下，游离氯对脊髓灰质炎病毒1的灭活作用在硼酸（0.05 M）-氢氧化钠缓冲液中比在纯化（经活性炭过滤、去离子）水中快三倍。因此，在游离氯浓度给定的情况下，使纯化水中相同比例的脊髓灰质炎病毒失活所需的时间是硼酸-氢氧化钠缓冲液中的三倍。相反，在给定时间内，使纯化水中一定比例的病毒失活所需的氯量是硼酸-氢氧化钠缓冲液中的三倍。在使用游离氯及其他氯化合物进行消毒研究时，几乎总是使用缓冲液来控制pH值。在这类消毒研究中使用缓冲液控制pH值可能会扭曲所得数据，并且至少对于含盐量低的水而言，可能会严重高估病毒的灭活速率。
