生长因子对培养的人成纤维细胞中透明质酸合成的影响。
Effect of growth factors on hyaluronan synthesis in cultured human fibroblasts.
作者信息
Heldin P, Laurent T C, Heldin C H
机构信息
Department of Medical and Physiological Chemistry, Uppsala, Sweden.
出版信息
Biochem J. 1989 Mar 15;258(3):919-22. doi: 10.1042/bj2580919.
The effect of various growth factors on the synthesis of hyaluronan in human fibroblasts was investigated. When tested in medium containing 0.5% fetal calf serum, platelet-derived growth factor (PDGF)-BB was found to stimulate hyaluronan synthesis; the maximal response was equal to or higher than that obtained with 10% fetal calf serum. PDGF-AA gave only a limited effect, indicating that the stimulatory effect of PDGF on hyaluronan synthesis was mainly transduced via the B-type PDGF receptor. Epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and transforming growth factor (TGF)-beta 1 also stimulated hyaluronan synthesis; their effects were less than that of PDGF-BB, but combinations of factors produced potent stimulatory effects on hyaluronan synthesis. All factors stimulated hyaluronan synthesis in sparse as well as dense cultures. The effects of the factors on hyaluronan synthesis did not correlate with their mitogenic activities; PDGF-BB, EGF and bFGF are equipotent mitogens, but PDGF-BB had a much more potent effect on hyaluronan synthesis, and TGF-beta actually inhibits the growth of fibroblasts under the conditions of the assay.
研究了多种生长因子对人成纤维细胞中透明质酸合成的影响。在含有0.5%胎牛血清的培养基中进行测试时，发现血小板衍生生长因子（PDGF）-BB能刺激透明质酸合成；最大反应等于或高于用10%胎牛血清获得的反应。PDGF-AA的作用有限，表明PDGF对透明质酸合成的刺激作用主要通过B型PDGF受体传导。表皮生长因子（EGF）、碱性成纤维细胞生长因子（bFGF）和转化生长因子（TGF）-β1也能刺激透明质酸合成；它们的作用小于PDGF-BB，但因子组合对透明质酸合成产生了强大的刺激作用。所有因子在稀疏和密集培养中均能刺激透明质酸合成。这些因子对透明质酸合成的作用与其促有丝分裂活性无关；PDGF-BB、EGF和bFGF是等效的促有丝分裂原，但PDGF-BB对透明质酸合成的作用要强得多，而在测定条件下TGF-β实际上抑制成纤维细胞的生长。
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