Williams Lynn B, Tessier Laurence, Koenig Judith B, Koch Thomas G
Department of Clinical Studies, University of Guelph, Guelph, Ontario, Canada.
Department of Biomedical Science, University of Guelph, Guelph, Ontario, Canada.
PLoS One. 2014 Dec 1;9(12):e113615. doi: 10.1371/journal.pone.0113615. eCollection 2014.
Multipotent mesenchymal stromal cells (MSC) are receiving increased attention for their non-progenitor immunomodulatory potential. Cryopreservation is commonly used for long-term storage of MSC. Post-thaw MSC proliferation is associated with a lag-phase in vitro. How this lag-phase affect MSC immunomodulatory properties is unknown. We hypothesized that in vitro there is no difference in lymphocyte suppression potential between quick-thawed cryopreserved equine cord blood (CB) MSC immediately included in mixed lymphocyte reaction (MLR) and same MSC allowed post-thaw culture time prior to inclusion in MLR. Cryopreserved CB-MSC from five unrelated foals were compared using two-way MLR. For each of the five unrelated MSC cultures, paired MLR assays of MSC allowed five days of post-thaw culture and MSC included in MLR assay immediately post-thawing were evaluated. We report no difference in the suppression of lymphocyte proliferation by CB-MSC that had undergone post-thaw culture and MSC not cultured post-thaw (p<0.0001). Also, there was no inter-donor variability between the lymphocyte suppressive properties of MSC harvested from the five different donors (p = 0.13). These findings suggest that cryopreserved CB-MSC may have clinical utility immediately upon thawing. One implication hereof is the possibility of using cryopreserved CB-MSC at third party locations without the need for cell culture equipment or competencies.
多能间充质基质细胞（MSC）因其非祖细胞免疫调节潜能而受到越来越多的关注。冷冻保存常用于MSC的长期储存。解冻后的MSC增殖与体外的延迟期相关。这种延迟期如何影响MSC的免疫调节特性尚不清楚。我们假设，在体外，快速解冻后立即用于混合淋巴细胞反应（MLR）的冷冻保存马脐带血（CB）MSC与解冻后先进行培养再用于MLR的相同MSC在淋巴细胞抑制潜能方面没有差异。使用双向MLR比较了来自五匹无关小马驹的冷冻保存CB-MSC。对于五种无关的MSC培养物中的每一种，评估了解冻后培养五天的MSC与解冻后立即用于MLR检测的MSC的配对MLR检测。我们报告称，解冻后培养的CB-MSC与解冻后未培养的MSC在抑制淋巴细胞增殖方面没有差异（p<0.0001）。此外，从五个不同供体收获的MSC的淋巴细胞抑制特性之间也没有供体间变异性（p = 0.13）。这些发现表明，冷冻保存的CB-MSC解冻后可能立即具有临床应用价值。由此带来的一个影响是，有可能在第三方地点使用冷冻保存的CB-MSC，而无需细胞培养设备或专业能力。
