College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China.
College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China.
Carbohydr Polym. 2015 Aug 1;126:17-22. doi: 10.1016/j.carbpol.2015.03.031. Epub 2015 Mar 21.
Preliminary characterization and antioxidant activity in vitro and in vivo investigation of the polysaccharide fraction named as RCSP II, which was extracted from Rana chensinensis skin, were performed. Results indicated that RCSP II comprised glucose, galactose, and mannose in a molar ratio of 87.82:2.77:1.54 with a molecular weight of 12.8 kDa. Antioxidant activity assay in vitro showed that RCSP II exhibited 75.2% scavenging activity against 2,2'-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) radicals at the concentration of 2500 mg/L and 85.1% against chelated ferrous ion at 4000 mg/L. Antioxidant activity assay in vivo further showed that RCSP II increased the activities of antioxidant enzymes, decreased the levels of malondialodehyde, and enhanced total antioxidant capabilities in livers and sera of d-galactose induced mice. These results suggested that RCSP II could have potential antioxidant applications as medicine or functional food.
对从中华大蟾蜍皮肤中提取的多糖 RCSP II 进行了初步的特性描述和体外、体内抗氧化活性研究。结果表明，RCSP II 由葡萄糖、半乳糖和甘露糖组成，摩尔比为 87.82:2.77:1.54，分子量为 12.8 kDa。体外抗氧化活性测定表明，RCSP II 在 2500 mg/L 浓度下对 2,2'-偶氮-双(3-乙基苯并噻唑啉-6-磺酸)自由基的清除率为 75.2%，在 4000 mg/L 浓度下对亚铁离子螯合的抑制率为 85.1%。体内抗氧化活性测定进一步表明，RCSP II 能提高 D-半乳糖诱导的小鼠肝脏和血清中抗氧化酶的活性，降低丙二醛的含量，增强总抗氧化能力。这些结果表明，RCSP II 作为药物或功能性食品具有潜在的抗氧化应用价值。
