Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai 90112, Thailand.
Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai 90112, Thailand; Center of Excellence on Hazardous Substance Management (HSM), Hat Yai, Thailand.
Carbohydr Polym. 2015 Jan 22;115:334-41. doi: 10.1016/j.carbpol.2014.08.099. Epub 2014 Sep 15.
Removal of Na(+) by binding with exopolymeric substances (EPS) from Rhodopseudomonas palustris TN114 and PP803 was investigated. The moderate negative correlation pairs (rp) between remaining Alcian blue and amount of Na(+) adsorbed on EPS from strains TN114 and PP803 were -0.652 and -0.609. Both strains showed positive relationships between the amounts of EPS produced and bacterial growth. EPS from strain PP803 had a higher efficiency in removing Na(+) than the EPS from strain TN114 based on their EC50 values (1.79 and 1.49 mg/mL for TN114 and PP803, respectively). The principal component from EPS of strain PP803 which was responsible for salt removal was purified and it was identified as a polysaccharide (≈18 kDa) mainly composed of galacturonic acid. Overall results suggested that EPS is a key factor that our strains used to bind Na(+) allowing their survival in high NaCl concentrations.
研究了从沼泽红假单胞菌 TN114 和 PP803 中通过与胞外聚合物（EPS）结合去除 Na(+)。TN114 和 PP803 菌株 EPS 上吸附的剩余阿尔辛蓝与 Na(+) 量之间存在中度负相关对（rp），分别为-0.652 和-0.609。两种菌株的 EPS 产量与细菌生长之间均呈正相关关系。基于 EC50 值（TN114 和 PP803 分别为 1.79 和 1.49 mg/mL），PP803 菌株的 EPS 去除 Na(+)的效率高于 TN114 菌株的 EPS。负责去除盐分的 PP803 菌株 EPS 的主要成分已被纯化，并被鉴定为一种多糖（约 18 kDa），主要由半乳糖醛酸组成。总体结果表明，EPS 是我们的菌株用于结合 Na(+)以在高 NaCl 浓度下生存的关键因素。
