Ezejiofor Anthoneth Ndidi, Orish Chinna Nneka, Orisakwe Orish Ebere
Faculty of Pharmaceutical Sciences, University of Port Harcourt, Rivers State, Nigeria.
Department of Anatomy, College of Health Sciences, University of Port Harcourt, Rivers State, Nigeria.
Chin J Nat Med. 2014 Oct;12(10):745-52. doi: 10.1016/S1875-5364(14)60114-X. Epub 2014 Oct 31.
Some plants have proven efficacy in the management of diabetes mellitus, of which Costus afer is one. This study was designed to evaluate the cytological and biochemical properties, and comparative ameliorating effects, of an aqueous extract of Costus afer Ker Gawl. (Costaceae) leaf and glibenclamide (GBM), in liver, kidney, and pancreatic injury induced by alloxan. Thirty male albino rats were divided into six weight-matched groups. Group one served as the negative control (non-induced and non-treated, control), while groups 2-6 were alloxan-induced diabetic groups. Group 2 served as a positive control (induced and non-treated, IC), groups 3-5 were treated with different doses of the extract (375, 750, and 1,125 mg/kg body weight) and glibenclamide, respectively. Body weight, absolute and relative organ weights, food and fluid intake, levels of serum glucose and liver enzymes and kidney parameters were calculated and compared. Hepatocytes, renal tubules, and pancreatic cells of diabetic rats, in diabetic non-treated and treated rats were harvested and examined histopathologically. There was dose dependent amelioration on the injuries induced by alloxan on both hepatocytes, renal tubules, and pancreatic cells after treatment with Costus afer. The glucose level was reduced significantly in the Costus afer treated diabetic rats compared with the non-treated diabetic group. Costus afer leaves seem to be effective against diabetic cell injury induced in rat liver, kidney, and pancreas.
一些植物已被证明对糖尿病的治疗有效，非洲闭鞘姜就是其中之一。本研究旨在评估非洲闭鞘姜（闭鞘姜科）叶水提取物和格列本脲（GBM）在四氧嘧啶诱导的肝、肾和胰腺损伤中的细胞学和生化特性以及比较改善作用。将30只雄性白化病大鼠分为六个体重匹配组。第一组作为阴性对照（未诱导且未治疗，对照），而第2 - 6组为四氧嘧啶诱导的糖尿病组。第2组作为阳性对照（诱导但未治疗，IC），第3 - 5组分别用不同剂量的提取物（375、750和1125毫克/千克体重）和格列本脲治疗。计算并比较体重、绝对和相对器官重量、食物和液体摄入量、血清葡萄糖水平、肝酶水平和肾脏参数。收集糖尿病未治疗和治疗大鼠的肝细胞、肾小管和胰腺细胞，并进行组织病理学检查。用非洲闭鞘姜治疗后，对四氧嘧啶诱导的肝细胞、肾小管和胰腺细胞损伤有剂量依赖性改善作用。与未治疗的糖尿病组相比，用非洲闭鞘姜治疗的糖尿病大鼠的血糖水平显著降低。非洲闭鞘姜叶似乎对大鼠肝脏、肾脏和胰腺中诱导的糖尿病细胞损伤有效。
