兔网织红细胞能量代谢的成熟依赖性变化。
Maturation-dependent changes of the rabbit reticulocyte energy metabolism.
作者信息
Kostić M M, Rapoport S M
机构信息
Institute of Physiology, Faculty of Medicine, University S. Markovic, Kragujevac, Yugoslavia.
出版信息
FEBS Lett. 1989 Jun 19;250(1):40-4. doi: 10.1016/0014-5793(89)80680-5.
Rabbit reticulocytes were separated into four fractions of different maturity in order to investigate the changes of cellular respiration and glycolysis, adenine nucleotides, 2,3-biphosphoglycerate (2,3-BPG) as well as cyclic AMP level during the transition from the youngest to the most mature reticulocytes. A significant reduction of total oxygen consumption, mainly due to depression of coupled respiration was found. The decline of respiration was accompanied by a 2-fold increase of the rate of aerobic glycolysis indicating a reduced Pasteur effect during maturation. A decline of ATP and an increase of ADP concentration was found. The oxygen-delivery capacity of the red cells increased by about 26% caused by an increase of the 2,3-BPG level of about 2 mmol/l cells. Cyclic AMP level in the fraction of youngest reticulocytes was about 60-fold higher than that in mature rabbit erythrocytes. The biggest decline of cyclic AMP was registered during the transition from youngest to the intermediate stage of maturity.
为了研究从最幼稚到最成熟的网织红细胞转变过程中细胞呼吸和糖酵解、腺嘌呤核苷酸、2,3-二磷酸甘油酸（2,3-BPG）以及环磷酸腺苷（cAMP）水平的变化，将兔网织红细胞分离为四个不同成熟度的组分。发现总氧消耗量显著降低，主要是由于偶联呼吸的抑制。呼吸的下降伴随着有氧糖酵解速率增加两倍，表明成熟过程中巴斯德效应降低。发现ATP下降而ADP浓度增加。由于2,3-BPG水平增加约2 mmol/l细胞，红细胞的氧输送能力增加约26%。最幼稚网织红细胞组分中的cAMP水平比成熟兔红细胞中的高约60倍。在从最幼稚到中等成熟阶段的转变过程中，cAMP下降幅度最大。
Zotero 插件