Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.
Department of Respiratory Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.
Gene. 2015 Jan 1;554(1):114-9. doi: 10.1016/j.gene.2014.10.038. Epub 2014 Oct 24.
Growing number of long intergenic noncoding RNAs (lincRNAs) have recently been identified in mammals as new modulators in cancer origination and progression involved in a broad range of biological processes. Long intergenic noncoding RNA POU3F3 (linc-POU3F3) has been characterized as a highly conserved functional transcription regulator in esophageal squamous cell carcinoma. The contributions of this lincRNA to glioblastoma remain unknown. In this present study, we investigated the expression pattern and functional role of linc-POU3F3 in glioma by using real-time PCR and gain-/loss-of-function studies. The results revealed that linc-POU3F3 levels were extraordinarily associated with the tumor WHO grade. In related biochemical assays, overexpression of linc-POU3F3 promotes cell viability and proliferation in glioma cells, whereas knockdown of linc-POU3F3 showed the opposite effect. As expected, we also found that linc-POU3F3 expression was negatively correlated with the mRNA level of POU3F3 (the evolutionarily conserved neighbor gene of linc-POU3F3). Our results indicate that linc-POU3F3 might affect glioma development via altering expression level of POU3F3, and lead us to believe that linc-POU3F3 may also have a crucial regulatory role in glioma progression.
越来越多的长链非编码 RNA（lincRNAs）最近在哺乳动物中被鉴定为涉及广泛生物过程的癌症起源和进展的新调节剂。长链非编码 RNA POU3F3（linc-POU3F3）已被表征为食管鳞状细胞癌中高度保守的功能转录调节因子。该 lincRNA 对神经胶质瘤的贡献尚不清楚。在本研究中，我们通过实时 PCR 和增益/损耗功能研究，研究了 linc-POU3F3 在神经胶质瘤中的表达模式和功能作用。结果表明，linc-POU3F3 水平与肿瘤 WHO 分级有很大关系。在相关的生化测定中，linc-POU3F3 的过表达促进神经胶质瘤细胞的活力和增殖，而 linc-POU3F3 的敲低则显示出相反的效果。正如预期的那样，我们还发现 linc-POU3F3 的表达与 linc-POU3F3 的进化保守邻居基因 POU3F3 的 mRNA 水平呈负相关。我们的结果表明，linc-POU3F3 可能通过改变 POU3F3 的表达水平影响神经胶质瘤的发展，并使我们相信 linc-POU3F3 也可能在神经胶质瘤的进展中具有重要的调节作用。
