Department of Hepatobiliary Surgery, Jining No.1 People's Hospital, Jining 272000, Shandong, China.
Department of General Surgery, Jining Traditional Chinese Medicine Hospital, Jining 272000, Shandong, China.
Biomed Pharmacother. 2018 Sep;105:683-689. doi: 10.1016/j.biopha.2018.06.006. Epub 2018 Jun 12.
Linc-POU3F3 showed an up-regulated tendency and functioned as tumor promoter in glioma, esophageal cancer and colorectal cancer. There was no report about the expression pattern and clinical value of linc-POU3F3 in hepatocellular carcinoma. Thus, the purpose of our study is to explore the clinical significance and biological role of linc-POU3F3 in hepatocellular carcinoma. Our results suggested that levels of linc-POU3F3 were dramatically increased in hepatocellular carcinoma tissues and cell lines compared with paired normal hepatic tissues and normal hepatic cell line, respectively. Levels of linc-POU3F3 were positively correlated with clinical stage, tumor size, vascular invasion and metastasis. Moreover, high-expression of linc-POU3F3 was an independent prognostic factor for hepatocellular carcinoma patients. The gain- and loss-of-function experiments showed that linc-POU3F3 expression significantly promoted tumor cell proliferation, migration and invasion. In addition, linc-POU3F3 expression was negatively correlated with POU3F3 mRNA and protein expressions in hepatocellular carcinoma tissues, and negatively regulated POU3F3 mRNA and protein expressions in hepatocellular carcinoma cells. In conclusion, our study supports the first evidence that linc-POU3F3 plays an oncogenic role in hepatocellular carcinoma, and represents a potential therapeutic strategy for hepatocellular carcinoma patients.
Linc-POU3F3 在胶质瘤、食管癌和结直肠癌中呈现上调趋势，并发挥肿瘤促进作用。目前尚无关于 linc-POU3F3 在肝癌中的表达模式和临床价值的报道。因此，我们的研究旨在探讨 linc-POU3F3 在肝癌中的临床意义和生物学作用。我们的研究结果表明，与配对的正常肝组织和正常肝细胞系相比，肝癌组织和细胞系中 linc-POU3F3 的水平显著升高。linc-POU3F3 的水平与临床分期、肿瘤大小、血管侵犯和转移呈正相关。此外，高表达 linc-POU3F3 是肝癌患者的独立预后因素。获得和缺失功能实验表明，linc-POU3F3 的表达显著促进了肿瘤细胞的增殖、迁移和侵袭。此外，在肝癌组织中，linc-POU3F3 的表达与 POU3F3 mRNA 和蛋白表达呈负相关，并负调控肝癌细胞中 POU3F3 mRNA 和蛋白的表达。总之，本研究首次证实 linc-POU3F3 在肝癌中发挥致癌作用，为肝癌患者提供了一种潜在的治疗策略。
