Yao Yi, Liu Rebecca, Shin Min Sun, Trentalange Mark, Allore Heather, Nassar Ala, Kang Insoo, Pober Jordan S, Montgomery Ruth R
Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, United States.
Department of Immunobiology, Yale University School of Medicine, New Haven, CT, United States.
J Immunol Methods. 2014 Dec 15;415:1-5. doi: 10.1016/j.jim.2014.10.010. Epub 2014 Nov 4.
Analysis of immune cell states is paramount to our understanding of the pathogenesis of a broad range of human diseases. Immunologists rely on fluorescence cytometry for cellular analysis, and while detection of 8 markers is now well established, the overlap of fluorescent signals limits efficiency. Mass cytometry or CyTOF (Cytometry by Time-Of-Flight) is a new technology for multiparameter single cell analysis that overcomes many limitations of fluorescence-based flow cytometry and can routinely detect as many as 40 markers per sample. This technology provides tremendous detail for cellular analysis of multiple cell populations simultaneously and is a powerful technique for translational investigations. Here we present reproducible detection of immune cell subsets starting with as few as 10,000 cells. Our study provides methods to employ CyTOF for small samples, which is especially relevant for investigation of limited patient biopsies in translational and clinical research.
免疫细胞状态分析对于我们理解多种人类疾病的发病机制至关重要。免疫学家依靠荧光细胞术进行细胞分析，虽然目前已能很好地检测8种标志物，但荧光信号的重叠限制了效率。质谱流式细胞术或飞行时间质谱细胞术（CyTOF）是一种用于多参数单细胞分析的新技术，它克服了基于荧光的流式细胞术的许多局限性，并且每个样本通常可以检测多达40种标志物。这项技术为同时对多个细胞群体进行细胞分析提供了极为详细的信息，是转化研究中的一项强大技术。在这里，我们展示了从低至10000个细胞开始对免疫细胞亚群进行可重复检测。我们的研究提供了将CyTOF应用于小样本的方法，这对于转化和临床研究中有限的患者活检样本的研究尤为重要。
