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十六种来自三种大型褐藻、、和的多糖组分的抗肿瘤活性比较分析。

A Comparative Analysis of the Anti-Tumor Activity of Sixteen Polysaccharide Fractions from Three Large Brown Seaweed, , and .

机构信息

Shandong Provincial Key Laboratory of Biochemical Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.

Wuqiong Food Co., Ltd., Raoping 515726, China.

出版信息

Mar Drugs. 2024 Jul 16;22(7):316. doi: 10.3390/md22070316.

DOI:10.3390/md22070316
PMID:39057425
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11278018/
Abstract

Searching for natural products with anti-tumor activity is an important aspect of cancer research. Seaweed polysaccharides from brown seaweed have shown promising anti-tumor activity; however, their structure, composition, and biological activity vary considerably, depending on many factors. In this study, 16 polysaccharide fractions were extracted and purified from three large brown seaweed species (, and ). The chemical composition analysis revealed that the polysaccharide fractions have varying molecular weights ranging from 8.889 to 729.67 kDa, and sulfate contents ranging from 0.50% to 10.77%. Additionally, they exhibit different monosaccharide compositions and secondary structures. Subsequently, their anti-tumor activity was compared against five tumor cell lines (A549, B16, HeLa, HepG and SH-SY5Y). The results showed that different fractions exhibited distinct anti-tumor properties against tumor cells. Flow cytometry and cytoplasmic fluorescence staining (Hoechst/AO staining) further confirmed that these effective fractions significantly induce tumor cell apoptosis without cytotoxicity. qRT-RCR results demonstrated that the polysaccharide fractions up-regulated the expression of , , , and while down-regulating the expression of and . This study comprehensively compared the anti-tumor activity of polysaccharide fractions from large brown seaweed, providing valuable insights into the potent combinations of brown seaweed polysaccharides as anti-tumor agents.

摘要

寻找具有抗肿瘤活性的天然产物是癌症研究的一个重要方面。褐藻多糖具有良好的抗肿瘤活性；然而，其结构、组成和生物活性因许多因素而异。在这项研究中，从三种大型褐藻（、和）中提取和纯化了 16 种多糖级分。化学组成分析表明，多糖级分的分子量范围为 8.889 至 729.67 kDa，硫酸根含量范围为 0.50%至 10.77%。此外，它们还具有不同的单糖组成和二级结构。随后，比较了它们对五种肿瘤细胞系（A549、B16、HeLa、HepG 和 SH-SY5Y）的抗肿瘤活性。结果表明，不同级分对肿瘤细胞表现出不同的抗肿瘤特性。流式细胞术和细胞质荧光染色（Hoechst/AO 染色）进一步证实，这些有效级分可显著诱导肿瘤细胞凋亡，而无细胞毒性。qRT-RCR 结果表明，多糖级分上调了 、 、 、 和 的表达，同时下调了 、 和 的表达。本研究全面比较了大型褐藻多糖级分的抗肿瘤活性，为褐藻多糖作为抗肿瘤药物的潜在组合提供了有价值的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/1a753152a684/marinedrugs-22-00316-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/792ecd5aab38/marinedrugs-22-00316-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/77a1012de22f/marinedrugs-22-00316-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/5cd59793d9dc/marinedrugs-22-00316-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/ecae7beb63fe/marinedrugs-22-00316-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/cdebd60f9a0f/marinedrugs-22-00316-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/286aa636b9d8/marinedrugs-22-00316-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/b4980ba84cb8/marinedrugs-22-00316-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/4b8966c4e860/marinedrugs-22-00316-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/5a219e6b1206/marinedrugs-22-00316-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/1a753152a684/marinedrugs-22-00316-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/792ecd5aab38/marinedrugs-22-00316-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/77a1012de22f/marinedrugs-22-00316-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/5cd59793d9dc/marinedrugs-22-00316-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/ecae7beb63fe/marinedrugs-22-00316-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/cdebd60f9a0f/marinedrugs-22-00316-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/286aa636b9d8/marinedrugs-22-00316-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/b4980ba84cb8/marinedrugs-22-00316-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/4b8966c4e860/marinedrugs-22-00316-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/5a219e6b1206/marinedrugs-22-00316-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7999/11278018/1a753152a684/marinedrugs-22-00316-g010.jpg

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