Yildiz U Hakan, Inci Fatih, Wang ShuQi, Toy Mehlika, Tekin H Cumhur, Javaid Asad, Lau Daryl T-Y, Demirci Utkan
Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Stanford University School of Medicine, Canary Center at Stanford for Cancer Early Detection, Palo Alto, CA 94304, United States.
Department of Surgery, Stanford School of Medicine, Palo Alto, CA, United States.
Biotechnol Adv. 2015 Jan-Feb;33(1):178-190. doi: 10.1016/j.biotechadv.2014.11.003. Epub 2014 Nov 20.
The control of hepatitis B virus (HBV) infection is a challenging task, specifically in developing countries there is limited access to diagnostics and antiviral treatment mainly due to high costs and insufficient healthcare infrastructure. Although the current diagnostic technologies can reliably detect HBV, they are relatively laborious, impractical and require expensive resources that are not suitable for resource-limited settings. Advances in micro/nanotechnology are pioneering the development of new generation methodologies in diagnosis and screening of HBV. Owing to combination of nanomaterials (metal/inorganic nanoparticles, carbon nanotubes, etc.) with microfabrication technologies, utilization of miniaturized sensors detecting HBV and other viruses from ultra-low volume of blood, serum and plasma is realized. The state-of-the-art microfluidic devices with integrated nanotechnologies potentially allow for inexpensive HBV screening at low cost. This review aims to highlight recent advances in nanotechnology and microfabrication processes that are employed for developing point-of-care (POC) HBV assays.
控制乙型肝炎病毒（HBV）感染是一项具有挑战性的任务，特别是在发展中国家，由于成本高昂和医疗基础设施不足，获得诊断和抗病毒治疗的机会有限。尽管当前的诊断技术能够可靠地检测出HBV，但它们相对繁琐、不实用，并且需要昂贵的资源，不适用于资源有限的环境。微/纳米技术的进步正在开创HBV诊断和筛查新一代方法的发展。由于纳米材料（金属/无机纳米颗粒、碳纳米管等）与微制造技术的结合，实现了利用微型传感器从超少量血液、血清和血浆中检测HBV及其他病毒。具有集成纳米技术的先进微流控设备有可能以低成本进行廉价的HBV筛查。本综述旨在突出纳米技术和微制造工艺在开发即时检测（POC）HBV检测方法方面的最新进展。
