Jia Fengjuan, Qi Shengdong, Li Hui, Liu Pu, Li Pengcheng, Wu Changai, Zheng Chengchao, Huang Jinguang
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
Biochem Biophys Res Commun. 2014 Nov 28;454(4):505-11. doi: 10.1016/j.bbrc.2014.10.136. Epub 2014 Nov 1.
Late embryogenesis abundant (LEA) proteins are implicated in various abiotic stresses in higher plants. In this study, we identified a LEA protein from Arabidopsis thaliana, AtLEA14, which was ubiquitously expressed in different tissues and remarkably induced with increased duration of salt treatment. Subcellular distribution analysis demonstrated that AtLEA14 was mainly localized in the cytoplasm. Transgenic Arabidopsis and yeast overexpressing AtLEA14 all exhibited enhanced tolerance to high salinity. The transcripts of salt stress-responsive marker genes (COR15a, KIN1, RD29B and ERD10) were overactivated in AtLEA14 overexpressing lines compared with those in wild type plants under normal or salt stress conditions. In vivo and in vitro analysis showed that AtLEA14 could effectively stabilize AtPP2-B11, an important E3 ligase. These results suggested that AtLEA14 had important protective functions under salt stress conditions in Arabidopsis.
胚胎发育晚期丰富（LEA）蛋白与高等植物中的各种非生物胁迫有关。在本研究中，我们从拟南芥中鉴定出一种LEA蛋白AtLEA14，它在不同组织中普遍表达，并随着盐处理时间的延长而显著诱导表达。亚细胞定位分析表明，AtLEA14主要定位于细胞质中。过表达AtLEA14的转基因拟南芥和酵母都表现出对高盐度的耐受性增强。与野生型植物相比，在正常或盐胁迫条件下，AtLEA14过表达株系中盐胁迫响应标记基因（COR15a、KIN1、RD29B和ERD10）的转录本被过度激活。体内和体外分析表明，AtLEA14可以有效地稳定一种重要的E3连接酶AtPP2-B11。这些结果表明，AtLEA14在拟南芥盐胁迫条件下具有重要的保护功能。
