Ciufolini M G, Maroli M, Verani P
Am J Trop Med Hyg. 1985 Jan;34(1):174-9. doi: 10.4269/ajtmh.1985.34.174.
Phlebotomus perniciosus were infected by intrathoracic inoculation and membrane feeding techniques with two phleboviruses (Toscana and Arbia) isolated in Italy from this sand fly species. Low levels of multiplication of both viruses were detected after intrathoracic inoculation of the sand flies. Only some insects were found infected after oral ingestion of the two viruses. The percentage of flies infected orally was related to the amount of virus ingested. Toscana virus was transovarially transmitted to two larvae of the F1 progeny of orally infected sand flies. No signs of infection were observed after oral infection when unnatural virus-vector combinations were tested, e.g., Toscana virus-P. papatasi or Naples sand fly fever virus-P. perniciosus. The virus concentrations recorded in P. perniciosus experimentally infected with both Toscana and Arbia viruses were similar to those found in naturally-infected sand flies.
通过胸腔接种和膜饲技术，用从意大利的这种白蛉分离出的两种白蛉病毒（托斯卡纳病毒和阿尔比亚病毒）感染了有害白蛉。在对白蛉进行胸腔接种后，检测到两种病毒的增殖水平较低。口服摄入这两种病毒后，仅发现部分昆虫被感染。经口感染的白蛉百分比与摄入的病毒量有关。托斯卡纳病毒经卵巢传播至经口感染白蛉的F1代的两只幼虫。当测试非自然的病毒-媒介组合时，如托斯卡纳病毒-巴氏白蛉或那不勒斯白蛉热病毒-有害白蛉，经口感染后未观察到感染迹象。在经实验感染托斯卡纳病毒和阿尔比亚病毒的有害白蛉中记录的病毒浓度与在自然感染的白蛉中发现的浓度相似。
