Sun Chang-Li, Ni Wei, Yan Huan, Liu Zhen-Hua, Yang Lian, Si Yong-Ai, Hua Yan, Chen Chang-Xiang, He Li, Zhao Jin-Hua, Liu Hai-Yang
Steroids. 2014 Dec;92:90-5. doi: 10.1016/j.steroids.2014.09.008.
In order to utilize and protect the resources of Rhizoma Paridis rationally, we carried out a phytochemical investigation on the non-medicinal (aerial) parts of Paris verticillata that led to the isolation of fifteen steroidal saponins. Among them, three are new spirostanol saponins, named parisverticosides A–C (1–3), as well as one new cholestane glycoside, named parisverticoside D (4). Their structures were elucidated by extensive spectroscopic analysis and acid hydrolysis. The aglycone of compound 1 is a new spirostane and identified as (23S,24S,25S)-spirost-5-en-1b,3b,23, 24-tetraol. The selected isolates were evaluated for induced platelet aggregation activity and compound 5 showed 62% maximal platelet aggregation rate at the concentration of 300 lg/mL.
为了合理利用和保护重楼资源，我们对滇重楼的非药用（地上）部分进行了植物化学研究，从中分离得到15个甾体皂苷。其中，有3个新的螺甾烷醇型皂苷，命名为滇重楼苷A - C（1 - 3），以及1个新的胆甾烷糖苷，命名为滇重楼苷D（4）。通过广泛的光谱分析和酸水解确定了它们的结构。化合物1的苷元是一种新的螺甾烷，鉴定为(23S,24S,25S)-螺甾-5-烯-1β,3β,23,24-四醇。对所选分离物进行了诱导血小板聚集活性评估，化合物5在浓度为300μg/mL时显示出62%的最大血小板聚集率。
