Aose Masamoto, Matsushima Hiroyuki, Mukai Koichiro, Katsuki Yoko, Gotoh Norihito, Senoo Tadashi
From the Department of Ophthalmology, Dokkyo Medical University, Tochigi, Japan.
From the Department of Ophthalmology, Dokkyo Medical University, Tochigi, Japan.
J Cataract Refract Surg. 2014 Dec;40(12):2128-33. doi: 10.1016/j.jcrs.2014.10.013. Epub 2014 Oct 25.
To evaluate whether and how intraocular lens (IOL) implantation influences the development of anterior capsule contraction and posterior capsule opacification (PCO).
Department of Ophthalmology, Dokkyo Medical University, Mibu, Tochigi, Japan.
Experimental study.
Phacoemulsification was performed in 8-week-old white rabbits. A hydrophobic acrylate IOL (12.5 mm) (YA-60BBR) was implanted in 1 eye and no IOL was implanted in the fellow eye. Slitlamp microscopy and anterior segment analysis were performed to evaluate anterior capsule contraction after the surgery. Four weeks postoperatively, sections of the eyes were made, and the thickness of the proliferated lens epithelial cell (LEC) layer at the posterior capsule was measured to assess the PCO. In addition, LECs from white rabbits were cultured in medium containing 50% aqueous humor or in medium containing 50% saline to determine the influence of the aqueous humor on LECs and to compare the degree of LEC proliferation.
Starting 2 weeks after surgery, anterior capsule contraction progressed more significantly in the IOL group than in the group without IOLs. Four weeks postoperatively, LEC thickness at the posterior capsule was significantly less in the group without IOLs than in the IOL group. In the culture study, LEC proliferation was more inhibited in the aqueous humor group than in the saline group.
Progression of anterior capsule contraction and PCO is less likely in aphakic eyes than in IOL-implanted eyes. The mechanism of prevention may involve aqueous humor-induced inhibition of LEC proliferation.
评估人工晶状体（IOL）植入是否以及如何影响前囊收缩和后囊混浊（PCO）的发展。
日本枥木县壬生市独协医科大学眼科。
实验研究。
对8周龄的白色家兔进行超声乳化手术。一只眼植入疏水丙烯酸酯IOL（12.5mm）（YA - 60BBR），对侧眼不植入IOL。术后进行裂隙灯显微镜检查和眼前节分析以评估前囊收缩情况。术后4周，制作眼球切片，测量后囊处增殖的晶状体上皮细胞（LEC）层的厚度以评估PCO。此外，将白色家兔的LEC在含有50%房水的培养基或含有50%盐水的培养基中培养，以确定房水对LEC的影响并比较LEC增殖程度。
术后2周开始，IOL组的前囊收缩比无IOL组进展更显著。术后4周，无IOL组后囊处的LEC厚度明显小于IOL组。在培养研究中，房水组的LEC增殖比盐水组受到更明显的抑制。
无晶状体眼比植入IOL的眼睛发生前囊收缩和PCO进展的可能性更小。预防机制可能涉及房水诱导的LEC增殖抑制。
