Zhang Keke, Zhu Xiangjia, Chen Minjie, Sun Xinghuai, Yang Jin, Zhou Peng, Lu Yi
Department of Ophthalmology, Eye and Ear, Nose, and Throat Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, 83 Fenyang Road, Shanghai 200031, China; Key Laboratory of Visual Impairment and Restoration of Shanghai, Fudan University, 83 Fenyang Road, Shanghai 200031, China.
Department of Ophthalmology, Parkway Health, Specialty and Inpatient Center (Luwan), 170 DanShui Road, Floor 3, Shanghai 200020, China; Parkway Health Hong Qiao Medical Center, 2258 Hong Qiao Road, Shanghai 200335, China.
J Ophthalmol. 2016;2016:5438676. doi: 10.1155/2016/5438676. Epub 2016 Jan 28.
Purpose. The purpose of the study was to elucidate the role of transforming growth factor-beta 2 (TGF-β2) in the development of high myopic capsular contraction syndrome. Methods. Nineteen cases of capsular contraction syndrome, including 14 with high myopia, were collected, and their clinical data were reviewed. Aqueous humor and anterior capsular membranes were obtained during capsulotomy. Hematoxylin-eosin and immunohistochemical staining with anti-TGF-β2 antibody were performed on capsular membranes. TGF-β2 levels in aqueous humor were assayed using ELISA and western blot. Results. High myopia was significantly associated with the incidence of capsular contraction syndrome (odds ratio: 14.74, P < 0.001, 95% CI: 5.29-41.05). Histopathological analysis revealed proliferation of fibroblast-like lens epithelial cells on the shrunken anterior capsule, labeled with TGF-β2 antibodies. ELISA and Western blot showed higher levels of TGF-β2 in aqueous humor of patients with capsular contraction syndrome and high myopia. Conclusions. High myopia is a risk factor for capsular contraction syndrome. Elevated TGF-β2 levels in high myopic cataract patients may play an important role in the pathogenesis of capsular contraction syndrome.
目的。本研究的目的是阐明转化生长因子β2（TGF-β2）在高度近视性晶状体后囊膜收缩综合征发生发展中的作用。方法。收集19例晶状体后囊膜收缩综合征患者，其中14例为高度近视患者，并回顾其临床资料。在晶状体切开术中获取房水和前囊膜。对囊膜进行苏木精-伊红染色及抗TGF-β2抗体免疫组化染色。采用酶联免疫吸附测定法（ELISA）和蛋白质印迹法检测房水中TGF-β2水平。结果。高度近视与晶状体后囊膜收缩综合征的发生率显著相关（比值比：14.74，P < 0.001，95%可信区间：5.29 - 41.05）。组织病理学分析显示，萎缩的前囊膜上有成纤维细胞样晶状体上皮细胞增殖，TGF-β2抗体标记阳性。ELISA和蛋白质印迹法显示，晶状体后囊膜收缩综合征合并高度近视患者房水中TGF-β2水平较高。结论。高度近视是晶状体后囊膜收缩综合征的一个危险因素。高度近视性白内障患者房水中TGF-β2水平升高可能在晶状体后囊膜收缩综合征的发病机制中起重要作用。
