Matsumoto T, Kumazawa J, van der Auwera P
Department of Urology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
J Urol. 1989 Aug;142(2 Pt 1):399-402. doi: 10.1016/s0022-5347(17)38772-4.
The effects of hyperosmolality induced by high concentrations of NaCl and urea on the functions of human polymorphonuclear leukocytes was examined. Phagocytosis, intracellular killing of bacteria and superoxide production were inhibited under hyperosmotic conditions. The mechanism of the inhibition of superoxide production was different for NaCl and urea. The inhibition by NaCl was indirect and abolished at 4C and the inhibition by urea was immediate, direct and not abolished at 4C. The intracellular ATP content of leukocytes under hyperosmotic conditions induced by NaCl was significantly reduced and this reduction was abolished at 4C. On the other hand, ATP content under such conditions induced with urea was not reduced. These results suggest that the inhibition of leukocyte function by NaCl is due to an exhaustion of energy source caused by an activation of Na-K pump, and the inhibition by urea is direct inactivation of leukocytes and not due to energy exhaustion.
研究了高浓度氯化钠和尿素诱导的高渗对人多形核白细胞功能的影响。在高渗条件下，吞噬作用、细胞内细菌杀伤和超氧化物生成均受到抑制。氯化钠和尿素抑制超氧化物生成的机制不同。氯化钠的抑制作用是间接的，在4℃时消失；尿素的抑制作用是即时、直接的，在4℃时不消失。在氯化钠诱导的高渗条件下，白细胞的细胞内ATP含量显著降低，且这种降低在4℃时消失。另一方面，在尿素诱导的这种条件下，ATP含量没有降低。这些结果表明，氯化钠对白细胞功能的抑制是由于钠钾泵激活导致能量来源耗尽，而尿素的抑制作用是白细胞的直接失活，并非由于能量耗尽。
