Matsumoto T, Takahashi K, Kubo S, Haraoka M, Mizunoe Y, Tanaka M, Ogata N, Naito S, Kumazawa J, Watanabe Y
Department of Urology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Urol Int. 1993;50(1):1-5. doi: 10.1159/000282438.
Chemotaxis is one of the most important functions of the polymorphonuclear leukocyte (PMN). In the host defense against pyelonephritis, the renal medulla is a site of interaction between bacteria and PMNs. At this site the osmotic pressure is elevated due to a high concentration of NaCl and urea. We evaluated the in vitro chemotactic activity of PMNs under the hyperosmolar conditions created by high concentrations of NaCl and urea. This activity was suppressed by the stimulation of opsonized zymosan and formyl-methionyl-leucyl-phenylalanine. The inhibition of chemotaxis was partially preserved by phosphoenolpyruvic acid (PEP), a precursor of adenosine triphosphate (ATP), in hyperosmolar NaCl but not in urea. The intracellular content of ATP was increased by supplementing the hyperosmolar NaCl with PEP. These observations suggest that inhibition of the chemotactic activity of PMNs is due to differing mechanisms for each NaCl and urea, and that PEP may protect the PMNs against hyperosmolar NaCl by maintaining ATP content.
趋化性是多形核白细胞（PMN）最重要的功能之一。在宿主抵御肾盂肾炎的过程中，肾髓质是细菌与PMN相互作用的部位。在这个部位，由于高浓度的氯化钠和尿素，渗透压升高。我们评估了在高浓度氯化钠和尿素产生的高渗条件下PMN的体外趋化活性。这种活性受到调理酵母聚糖和甲酰甲硫氨酰亮氨酰苯丙氨酸刺激的抑制。在高渗氯化钠中，磷酸烯醇丙酮酸（PEP）（三磷酸腺苷（ATP）的前体）可部分保留对趋化性的抑制作用，但在尿素中则不然。通过向高渗氯化钠中补充PEP，ATP的细胞内含量增加。这些观察结果表明，PMN趋化活性的抑制是由于氯化钠和尿素各自不同的机制所致，并且PEP可能通过维持ATP含量来保护PMN免受高渗氯化钠的影响。
