Hampton M B, Chambers S T, Vissers M C, Winterbourn C C
Department of Pathology, Christchurch School of Medicine, New Zealand.
J Infect Dis. 1994 Apr;169(4):839-46. doi: 10.1093/infdis/169.4.839.
The effects of hypertonic sodium chloride and urea solutions on the phagocytosis and killing of Escherichia coli by neutrophils were examined. Neutrophil function was progressively inhibited by increasing osmolarity, with sodium chloride more inhibitory than urea. However, neutrophils killed at 25% of normal rates in 500 mosmol/L NaCl, and in 300 mosmol/L NaCl with 800 mosmol/L urea. These concentrations would rarely be exceeded in the human urinary tract. Inhibition of neutrophil superoxide production and degranulation was similar to that observed with phagocytosis and killing. Neutrophils also phagocytosed and killed E. coli in isotonic and hypertonic urine, but no improvement in function attributable to the uptake of urinary osmoprotectants was evident. It is concluded that physiologic levels of sodium chloride and urea will slow, but not prevent, neutrophil activity at a hypertonic site of infection.
研究了高渗氯化钠和尿素溶液对中性粒细胞吞噬和杀灭大肠杆菌的影响。随着渗透压升高，中性粒细胞功能逐渐受到抑制，氯化钠的抑制作用比尿素更强。然而，在500毫渗摩尔/升氯化钠以及300毫渗摩尔/升氯化钠与800毫渗摩尔/升尿素的混合溶液中，中性粒细胞的杀菌速率仅为正常速率的25%。这些浓度在人类尿道中很少会超过。中性粒细胞超氧化物生成和脱颗粒的抑制情况与吞噬和杀菌时观察到的相似。中性粒细胞也能在等渗和高渗尿液中吞噬和杀灭大肠杆菌，但未发现因摄取尿液中的渗透保护剂而使功能得到改善。结论是，生理水平的氯化钠和尿素会减缓但不会阻止中性粒细胞在高渗感染部位的活性。
