Matsumoto T, van der Auwera P, Watanabe Y, Tanaka M, Ogata N, Naito S, Kumazawa J
Department of Urology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Urol Res. 1991;19(4):223-7. doi: 10.1007/BF00305299.
Hyperosmolarity in the urinary tract inhibits the host defense against bacterial infection. NaCl contributes most to osmolarity in the renal medulla and urine. Therefore, we studied the effect of hyperosmolar NaCl on neutrophil function. When osmolarity was increased by NaCl, polymorphonuclear leukocytes (PMNL) became defective in phagocytosis, intracellular killing of bacteria, chemotactic activity, and superoxide production. This coincided with a decrease in the intracellular adenosine triphosphate (ATP) content of PMNL. Both the inhibition of superoxide production and the reduction in ATP content did not occur at 4 degrees C. By increasing ATP content, phosphoenol pyruvic acid (PEP) protected against the decrease in superoxide production. These results suggest that leukocyte function is inhibited by high concentrations of NaCl due to the activation of the Na(+)-K+ pump. PEP, an ATP precursor, can protect PMNL against osmotic stress by raising the intracellular concentration of ATP.
尿路中的高渗状态会抑制宿主对细菌感染的防御能力。氯化钠对肾髓质和尿液的渗透压贡献最大。因此，我们研究了高渗氯化钠对中性粒细胞功能的影响。当通过氯化钠提高渗透压时，多形核白细胞（PMNL）在吞噬作用、细胞内细菌杀伤、趋化活性和超氧化物产生方面出现缺陷。这与PMNL细胞内三磷酸腺苷（ATP）含量的降低相吻合。超氧化物产生的抑制和ATP含量的降低在4℃时均未发生。通过增加ATP含量，磷酸烯醇丙酮酸（PEP）可防止超氧化物产生的减少。这些结果表明，由于钠钾泵的激活，高浓度氯化钠会抑制白细胞功能。PEP作为一种ATP前体，可通过提高细胞内ATP浓度来保护PMNL免受渗透压应激。
