Granato Alessandra, Renwick Simone, Yau Christopher, Kong Tiffany, Daigneault Michelle C, Knip Mikael, Allen-Vercoe Emma, Danska Jayne S
Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Dept. of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada.
Microbiome. 2024 Dec 5;12(1):255. doi: 10.1186/s40168-024-01976-w.
The human gut microbiota is inoculated at birth and undergoes a process of assembly and diversification during the first few years of life. Studies in mice and humans have revealed associations between the early-life gut microbiome and future susceptibility to immune and metabolic diseases. To resolve microbe and host contributing factors to early-life development and to disease states requires experimental platforms that support reproducible, longitudinal, and high-content analyses.
Here, we deployed a continuous single-stage chemostat culture model of the human distal gut to study gut microbiota from 18- to 24-month-old children integrating both culture-dependent and -independent methods. Chemostat cultures recapitulated multiple aspects of the fecal microbial ecosystem enabling investigation of relationships between bacterial strains and metabolic function, as well as a resource from which we isolated and curated a diverse library of early life bacterial strains.
We report the reproducible, longitudinal dynamics of early-life bacterial communities cultured in an advanced model of the human gut providing an experimental approach and a characterized bacterial resource to support future investigations of the human gut microbiota in early childhood.
人类肠道微生物群在出生时就已接种，并在生命的最初几年经历组装和多样化的过程。对小鼠和人类的研究揭示了早期肠道微生物组与未来对免疫和代谢疾病易感性之间的关联。要解析微生物和宿主对早期发育及疾病状态的影响因素，需要支持可重复、纵向和高内涵分析的实验平台。
在此，我们采用了一种人类远端肠道的连续单级恒化器培养模型，结合依赖培养和不依赖培养的方法，研究18至24个月大儿童的肠道微生物群。恒化器培养重现了粪便微生物生态系统的多个方面，能够研究细菌菌株与代谢功能之间的关系，同时也是我们从中分离和整理出不同早期生命细菌菌株文库的一个来源。
我们报告了在人类肠道的先进模型中培养的早期生命细菌群落的可重复纵向动态，提供了一种实验方法和一个经过表征的细菌资源，以支持未来对幼儿期人类肠道微生物群的研究。
