Rando R F, Lindheim S, Hasty L, Sedlacek T V, Woodland M, Eder C
Department of Pathology, Pennsylvania Hospital, Philadelphia 19107.
Am J Obstet Gynecol. 1989 Jul;161(1):50-5. doi: 10.1016/0002-9378(89)90231-7.
Exfoliated cells of the uterine cervix obtained from women during pregnancy and at the time of their first postpartum examination were used to monitor the prevalence of human papillomavirus infections in this population and to study the natural fluctuations in viral expression. When deoxyribonucleic acid hybridization analysis alone was used to monitor the presence of human papillomavirus infection, 20.9% of our study population had results that were positive for human papillomavirus deoxyribonucleic acid during their first-trimester examinations. A dramatic increase in the percentage of women with positive results for human papillomavirus deoxyribonucleic acid was observed at the time of the patients' third-trimester examinations (46%). The overall increase in human papillomavirus-positive patients was a combination of a small number of patients who had positive results on their first examination and negative results on their second examination, and a larger number of patients who had negative results on their first-trimester examination and positive results for human papillomavirus deoxyribonucleic acid in the exfoliated cervical cells at the time of their third-trimester examination. The total percentage of patients with positive results for human papillomavirus deoxyribonucleic acid in their cervical cells at one or both assay points during pregnancy was 52.5%. Samples obtained at the postpartum examination demonstrated a dramatic decrease in the number of samples positive for human papillomavirus deoxyribonucleic acid (17.5%). This result was a combination of a large decrease in human papillomavirus-positive patients coupled with a small increase in detectable levels of human papillomavirus deoxyribonucleic acid in cervical samples from patients who had negative results on their previous examination. This study demonstrates a very high level of detectable human papillomavirus deoxyribonucleic acid in exfoliated cervical cells obtained during pregnancy and shows that the detectable levels of human papillomavirus deoxyribonucleic acid fluctuate during pregnancy.
从孕期女性及其首次产后检查时获取的子宫颈脱落细胞，用于监测该人群中人类乳头瘤病毒感染的流行情况，并研究病毒表达的自然波动。当仅使用脱氧核糖核酸杂交分析来监测人类乳头瘤病毒感染的存在时，在我们的研究人群中，20.9%的女性在孕早期检查时人类乳头瘤病毒脱氧核糖核酸检测结果呈阳性。在患者孕晚期检查时，观察到人类乳头瘤病毒脱氧核糖核酸检测结果呈阳性的女性比例急剧上升（46%）。人类乳头瘤病毒阳性患者的总体增加是由少数首次检查结果为阳性而第二次检查结果为阴性的患者，以及大量孕早期检查结果为阴性但孕晚期检查时子宫颈脱落细胞中人类乳头瘤病毒脱氧核糖核酸检测结果为阳性的患者共同导致的。在孕期一个或两个检测点，子宫颈细胞中人类乳头瘤病毒脱氧核糖核酸检测结果呈阳性的患者总比例为52.5%。产后检查获取的样本显示，人类乳头瘤病毒脱氧核糖核酸检测阳性的样本数量大幅下降（17.5%）。这一结果是人类乳头瘤病毒阳性患者大幅减少，以及之前检查结果为阴性的患者子宫颈样本中可检测到的人类乳头瘤病毒脱氧核糖核酸水平略有上升共同作用的结果。这项研究表明，孕期获取的子宫颈脱落细胞中可检测到的人类乳头瘤病毒脱氧核糖核酸水平非常高，并且显示出人类乳头瘤病毒脱氧核糖核酸的可检测水平在孕期会波动。
