Sedlacek T V, Lindheim S, Eder C, Hasty L, Woodland M, Ludomirsky A, Rando R F
Department of Obstetrics and Gynecology, Pennsylvania Hospital, Philadelphia 19107.
Am J Obstet Gynecol. 1989 Jul;161(1):55-9. doi: 10.1016/0002-9378(89)90232-9.
We attempted to investigate mechanisms, in addition to sexual contact, by which human papillomaviruses associated with anogenital tract lesions could be transmitted. Samples of exfoliated cervical cells were obtained from 45 pregnant women and were assayed by Southern blot hybridization analysis for the presence of human papillomavirus nucleic acids. Twenty-five of the 45 women had cells positive for human papillomavirus deoxyribonucleic acid. A neonatal nasopharyngeal aspirate was obtained at term and analyzed for the presence of human papillomavirus deoxyribonucleic acid. We documented the presence of human papillomavirus deoxyribonucleic acid in the oral pharyngeal cavity of the neonates in 15 of 45 nasopharyngeal samples analyzed. Amniotic fluid was obtained from 13 patients when their membranes were artificially ruptured. These samples were assayed for the presence of human papillomavirus deoxyribonucleic acid; two of the 13 amniotic fluid samples contained human papillomavirus deoxyribonucleic acid. The detection of human papillomavirus deoxyribonucleic acid in the oral cavity of neonates is indicative of a perinatal mechanism of viral transmission. The detection of human papillomavirus deoxyribonucleic acid in the amniotic fluid may suggest an in utero mechanism of transmission. However, problems encountered in collecting the amniotic fluid samples preclude us from definitive interpretation of these data.
我们试图研究除性接触外，与肛门生殖道病变相关的人乳头瘤病毒的传播机制。从45名孕妇中获取脱落的宫颈细胞样本，通过Southern印迹杂交分析检测人乳头瘤病毒核酸的存在。45名女性中有25名的细胞中人乳头瘤病毒脱氧核糖核酸呈阳性。在足月时获取新生儿鼻咽吸出物，并分析其中人乳头瘤病毒脱氧核糖核酸的存在情况。在分析的45份鼻咽样本中，我们记录到15份新生儿口腔咽部存在人乳头瘤病毒脱氧核糖核酸。从13名患者人工破膜时获取羊水样本。检测这些样本中人乳头瘤病毒脱氧核糖核酸的存在情况；13份羊水样本中有2份含有该病毒。在新生儿口腔中检测到人乳头瘤病毒脱氧核糖核酸表明存在病毒围产期传播机制。在羊水中检测到人乳头瘤病毒脱氧核糖核酸可能提示存在宫内传播机制。然而，收集羊水样本时遇到的问题使我们无法对这些数据进行确切解读。
