Sen Subhrojit
Department of Viral Associated Human Cancer, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata, 700026, India.
Virus Genes. 2024 Jun;60(3):263-274. doi: 10.1007/s11262-024-02070-x. Epub 2024 Apr 25.
The investigation of perinatal transmission of HPV is vital for early screening of cervical/oral cancers. Here, transmission of HPV from the pregnant women to their infants was studied. p53 and Bcl-2 expressions and their correlations with HPV infection were examined. HPV infection was detected in the cervical and oral swabs of 135 mother-baby pairs employing both PCR and HC-II methods. 1 year follow-up with an interim visit at 3 months for mothers and 6 months for babies was performed. Immunocytochemistry of p53 and Bcl-2 using the streptavidin-biotin peroxidase method was performed. Prevalence of HPV infection in the mothers was 28.14%, (38/135) and 30.37% (41/135) determined by the PCR and HC-II methods respectively. HPV 16 and/or 18 was identified in 81.57% (31/38) and 82.92% (34/41) of the HPV + women estimated by PCR and HC-II methods respectively. Prevalence rate of HPV 16 among the HPV + pregnant women was 63.15% (24/38) and 65.85% (27/41) determined by PCR and HC-II methods respectively. The frequency of perinatal transmission was 21.05% (8/38) and 21.95% (9/41) determined by PCR and HC-II methods respectively at birth. The HPV + infants in the follow up study cleared the infection within 6 weeks. An abnormal nuclear expression of p53 and cytoplasmic expression of Bcl-2 were observed in the HPV + mother-baby pairs. Cesarean section did not protect the infants against perinatal HPV transmission. The detection of p53 and Bcl-2 proteins in the HPV + mother-baby pairs suggests that these biomarkers may be important in the early screening of oral/cervix cancers in positive cases.
人乳头瘤病毒（HPV）围产期传播的研究对于宫颈癌/口腔癌的早期筛查至关重要。在此，对HPV从孕妇传播至其婴儿的情况进行了研究。检测了p53和Bcl-2的表达及其与HPV感染的相关性。采用聚合酶链反应（PCR）和杂交捕获二代（HC-II）方法，在135对母婴的宫颈和口腔拭子中检测到了HPV感染。对母亲进行为期1年的随访，并在3个月时进行中期访视，对婴儿在6个月时进行中期访视。采用链霉亲和素-生物素过氧化物酶法对p53和Bcl-2进行免疫细胞化学检测。通过PCR和HC-II方法分别测定母亲中HPV感染的患病率为28.14%（38/135）和30.37%（41/135）。通过PCR和HC-II方法分别估计，在HPV阳性女性中，HPV 16和/或18的检出率分别为81.57%（31/38）和82.92%（34/41）。通过PCR和HC-II方法分别测定，HPV阳性孕妇中HPV 16的患病率分别为63.15%（24/38）和65.85%（27/41）。出生时，通过PCR和HC-II方法分别测定围产期传播的频率为21.05%（8/38）和21.95%（9/41）。在随访研究中，HPV阳性婴儿在6周内清除了感染。在HPV阳性母婴对中观察到p53的异常核表达和Bcl-2的细胞质表达。剖宫产并不能保护婴儿免受围产期HPV传播。在HPV阳性母婴对中检测p53和Bcl-2蛋白表明，这些生物标志物可能在阳性病例的口腔/宫颈癌早期筛查中具有重要意义。
