Duan Dongmei, Chen Liping, Yang Xiuyan, Tu Ya, Jiao Shuang
Department of Health Care, General Hospital of People's Liberation Army, Beijing, 100853, China,
J Nat Med. 2015 Apr;69(2):191-7. doi: 10.1007/s11418-014-0878-0. Epub 2014 Dec 3.
Depressive order is one of the most common psychiatric diseases, and Toona ciliata Roem. var. yunnanensis has shown many bioactivities in folk medicine. This study was designed to investigate the antidepressant-like effect of essential oil isolated from T. ciliata Roem. var. yunnanensis. Gas chromatography and mass spectrometry (GC-MS) was used to analyze the compositions of essential oil. The immobility time in the forced swimming test (FST), tail suspending test (TST), and open field test (OFT) were used to evaluate the antidepressive effects of essential oil. Furthermore, chronic mild stress (CMS) rats were established, and contents of dopamine (DA), norepinephrine (NE), and 5-hydroxytryptamine (5-HT) in the brain were determined by high-performance liquid chromatography-electron capture detector (HPLC-ECD). Western blotting was performed to investigate the effects of essential oil on the expressions of brain-derived neurotrophic factor (BDNF) protein in rats' brain. The GC-MS analysis showed that the main components of essential oil were estragole (6.16 %), β-elemene (24.91 %), β-cubebene (14.29 %), and γ-elemene (8.05 %). The results from the FST and TST demonstrated that the immobility time could be significantly reduced by essential oil (10, 20, 40, and 80 mg/kg), without accompanying changes in ambulation when assessed in the OFT. Additionally, the contents of DA, NE, 5-HT, and BDNF in the hippocampus of CMS rats could be increased by treatment with essential oil at doses of 20, 40, and 80 mg/kg. All these results suggested that essential oil could be considered as a new candidate for curing depressive disorders.
抑郁症是最常见的精神疾病之一，而云南香椿在民间医学中已显示出多种生物活性。本研究旨在探讨从云南香椿中分离得到的挥发油的抗抑郁样作用。采用气相色谱-质谱联用（GC-MS）分析挥发油的成分。通过强迫游泳试验（FST）、悬尾试验（TST）和旷场试验（OFT）中的不动时间来评估挥发油的抗抑郁作用。此外，建立慢性轻度应激（CMS）大鼠模型，采用高效液相色谱-电子捕获检测器（HPLC-ECD）测定大脑中多巴胺（DA）、去甲肾上腺素（NE）和5-羟色胺（5-HT）的含量。通过蛋白质免疫印迹法研究挥发油对大鼠脑中脑源性神经营养因子（BDNF）蛋白表达的影响。GC-MS分析表明，挥发油的主要成分是草蒿脑（6.16%）、β-榄香烯（24.91%）、β-荜澄茄烯（14.29%）和γ-榄香烯（8.05%）。FST和TST的结果表明，挥发油（10、20、40和80 mg/kg）可显著缩短不动时间，在OFT中评估时，行走情况无伴随变化。此外，20、40和80 mg/kg剂量的挥发油处理可增加CMS大鼠海马中DA、NE、5-HT和BDNF的含量。所有这些结果表明，挥发油可被视为治疗抑郁症的新候选药物。
