孢子光产物裂合酶：已知的、有争议的及未知的方面
Spore photoproduct lyase: the known, the controversial, and the unknown.
作者信息
Yang Linlin, Li Lei
机构信息
From the Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis (IUPUI), Indianapolis, Indiana, 46202 and.
From the Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis (IUPUI), Indianapolis, Indiana, 46202 and Department of Biochemistry and Molecular Biology, Indiana University School of Medicine (IUSM), Indianapolis, Indiana 46202
出版信息
J Biol Chem. 2015 Feb 13;290(7):4003-9. doi: 10.1074/jbc.R114.573675. Epub 2014 Dec 4.
Abstract
Spore photoproduct lyase (SPL) repairs 5-thyminyl-5,6-dihydrothymine, a thymine dimer that is also called the spore photoproduct (SP), in germinating endospores. SPL is a radical S-adenosylmethionine (SAM) enzyme, utilizing the 5'-deoxyadenosyl radical generated by SAM reductive cleavage reaction to revert SP to two thymine residues. Here we review the current progress in SPL mechanistic studies. Protein radicals are known to be involved in SPL catalysis; however, how these radicals are quenched to close the catalytic cycle is under debate.
摘要
孢子光产物裂解酶（SPL）可修复发芽内生孢子中的5-胸腺嘧啶基-5,6-二氢胸腺嘧啶，这是一种胸腺嘧啶二聚体，也被称为孢子光产物（SP）。SPL是一种自由基S-腺苷甲硫氨酸（SAM）酶，利用SAM还原裂解反应产生的5'-脱氧腺苷自由基将SP还原为两个胸腺嘧啶残基。在此，我们综述了SPL机制研究的当前进展。已知蛋白质自由基参与SPL催化；然而，这些自由基如何淬灭以完成催化循环仍存在争议。
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