Ruszczycky Mark W, Liu Hung-Wen
Division of Chemical Biology & Medicinal Chemistry, College of Pharmacy, University of Texas at Austin, Austin, Texas 78712, United States.
Department of Chemistry, University of Texas at Austin, Austin, Texas 78712, United States.
Biochemistry. 2024 Dec 17;63(24):3161-3183. doi: 10.1021/acs.biochem.4c00518. Epub 2024 Dec 3.
Radical -adenosyl-l-methionine (SAM) enzymes catalyze radical mediated chemical transformations notable for their diversity. The radical mediated reactions that take place in their catalytic cycles can be characterized with respect to one or more phases of initiation, propagation, and termination. Mechanistic models abound regarding these three phases of catalysis being regularly informed and updated by new discoveries that offer insights into their detailed workings. However, questions continue to be raised that touch on fundamental aspects of their mechanistic enzymology. Radical SAM enzymes are consequently far from fully understood, and this Perspective aims to outline some of the current models of radical SAM chemistry with an emphasis on lines of investigation that remain to be explored.
自由基 - 腺苷 - L - 甲硫氨酸（SAM）酶催化以其多样性而闻名的自由基介导的化学转化。在其催化循环中发生的自由基介导反应可根据引发、传播和终止的一个或多个阶段进行表征。关于催化的这三个阶段的机制模型众多，并且会定期根据新发现进行补充和更新，这些新发现为其详细作用机制提供了见解。然而，关于其机制酶学的基本方面仍不断有问题被提出。因此，自由基SAM酶远未被完全理解，本综述旨在概述一些当前的自由基SAM化学模型，重点关注仍有待探索的研究方向。
