Quiney R E, Wells M, Lewis F A, Terry R M, Michaels L, Croft C B
Department of Otolaryngology, Royal National Throat, Nose and Ear Hospital, London.
J Clin Pathol. 1989 Jul;42(7):694-8. doi: 10.1136/jcp.42.7.694.
A technique using a biotin-streptavidin polyalkaline phosphatase complex was applied to routinely fixed and processed biopsy specimens of laryngeal papillomata from 45 patients taken over the past 20 years to detect human papilloma virus (HPV) types 6 and 11. Two thirds of both adult and juvenile onset cases were positive for HPV 6 or HPV 11 or both. Five specimens of normal vocal cord epithelium were negative for HPV 6 and 11. The detailed clinical history, endoscopic findings, success of treatment and eventual prognosis were compared with the HPV state of biopsy material for each patient. Patients with multiple confluent lesions when first seen, whose histology showed florid koilocytosis and who had strongly positive reactivity for HPV 6 or 11 present in the surface epithelial cell nuclei, had a poor prognosis requiring multiple endoscopies to control their disease.
一种使用生物素-链霉亲和素多碱性磷酸酶复合物的技术，应用于过去20年中采集的45例喉乳头状瘤患者的常规固定和处理的活检标本，以检测6型和11型人乳头瘤病毒（HPV）。成人和青少年发病病例中，三分之二的病例HPV 6或HPV 11呈阳性，或两者均呈阳性。5例正常声带上皮标本HPV 6和11呈阴性。将每位患者的详细临床病史、内镜检查结果、治疗效果及最终预后与其活检材料的HPV状态进行比较。初诊时患有多个融合性病变、组织学显示显著挖空细胞增多且表面上皮细胞核中HPV 6或11反应性强阳性的患者，预后较差，需要多次内镜检查来控制病情。
