Price P, Winter J G, Shellam G R
Department of Microbiology, University of Western Australia, Nedlands.
Arch Virol. 1989;106(1-2):35-50. doi: 10.1007/BF01311036.
Adherent suppressor cells have often been implicated in the depression of immunocompetence following CMV infections. We have reported that high levels of cytostatic macrophages in the peritoneal cavities of infected mice correlate with genetically-based sensitivity to CMV disease, suggesting they may modulate protective immune responses. This study investigates the properties and kinetics of such cells. Genetically-susceptible BALB/c mice infected with MCMV accumulated activated peritoneal macrophages, 7 days post-infection. These cells suppressed 3H-thymidine-incorporation and lymphokine production in syngeneic lymphocyte cultures and hence appeared to have depressed accessory cell function, although interleukin-1 production and the capacity to take up colloidal gold were enhanced. The cytostatic activity was located in a low density fraction (1.05 g/ml), which was expanded by MCMV infection. The lowest density cells had higher frequencies of infection but the proportion of cells releasing virus (less than 0.2%) was below the proportion activated, as shown by the shift in the density profile or enhanced colloidal gold uptake. A comparable accumulation of cytostatic activated peritoneal macrophages occurred in mice treated with cyclosporine A, but nude mice showed macrophage activation without cytostasis, so the role of T cells is not resolved. The spleens of infected mice maintaining high levels of virus in this organ atrophied, and the remaining cells were unable to proliferate in culture. In contrast, mice clearing the virus developed splenomegaly and restricted responsiveness, which may be governed by cytostatic cells equivalent to those in the peritoneal cavity. The spread of virus to the lymph nodes was limited and MCMV-primed cells were readily demonstrable.
黏附性抑制细胞常被认为与巨细胞病毒（CMV）感染后免疫能力的降低有关。我们曾报道，感染小鼠腹腔中高水平的细胞抑制性巨噬细胞与基于遗传的对CMV疾病的易感性相关，这表明它们可能调节保护性免疫反应。本研究调查此类细胞的特性和动力学。感染鼠巨细胞病毒（MCMV）的遗传易感BALB/c小鼠在感染后7天积累了活化的腹腔巨噬细胞。这些细胞抑制了同基因淋巴细胞培养物中3H-胸腺嘧啶核苷的掺入和淋巴因子的产生，因此似乎具有降低的辅助细胞功能，尽管白细胞介素-1的产生和摄取胶体金的能力增强。细胞抑制活性位于低密度组分（1.05 g/ml）中，该组分因MCMV感染而扩增。最低密度的细胞感染频率较高，但释放病毒的细胞比例（低于0.2%）低于活化细胞的比例，这通过密度分布的变化或增强的胶体金摄取得以显示。用环孢素A处理的小鼠出现了类似的细胞抑制性活化腹腔巨噬细胞的积累，但裸鼠显示巨噬细胞活化但无细胞抑制作用，因此T细胞的作用尚未明确。在该器官中维持高水平病毒的感染小鼠的脾脏萎缩，其余细胞在培养中无法增殖。相反，清除病毒的小鼠出现脾肿大和反应性受限，这可能由与腹腔中类似的细胞抑制性细胞控制。病毒向淋巴结的传播有限，且易于证明存在MCMV致敏细胞。
