来自八放珊瑚红软柳珊瑚的多氧化甾体及其细胞毒性活性的体外评估。

Moritz Maria Izabel G, Marostica Lucas Lourenço, Bianco Everson M, Almeida Maria Tereza R, Carraro João L, Cabrera Gabriela M, Palermo Jorge A, Simões Cláudia M O, Schenkel Eloir P

Post-Graduation Program in Pharmacy, Federal University of Santa Catarina, Florianópolis, SC 88040-900, Brazil.

Invertebrates Department, National Museum, Federal University of Rio de Janeiro, Rio de Janeiro 20940-040, RJ, Brazil.

Mar Drugs. 2014 Dec 4;12(12):5864-80. doi: 10.3390/md12125864.

Five new polyoxygenated marine steroids-punicinols A-E (1-5)-were isolated from the gorgonian Leptogorgia punicea and characterized by spectroscopic methods (IR, MS, 1H, 13C and 2-D NMR). The five compounds induced in vitro cytotoxic effects against lung cancer A549 cells, while punicinols A and B were the most active, with IC50 values of 9.7 μM and 9.6 μM, respectively. The synergistic effects of these compounds with paclitaxel, as well as their effects on cell cycle distribution and their performance in the clonogenic assay, were also evaluated. Both compounds demonstrated significant synergistic effects with paclitaxel.

从柳珊瑚红瘦柳珊瑚（Leptogorgia punicea）中分离出5种新的多氧化海洋甾体化合物——石榴醇A - E（1 - 5），并通过光谱方法（红外光谱、质谱、1H、13C和二维核磁共振）对其进行了表征。这5种化合物对肺癌A549细胞具有体外细胞毒性作用，其中石榴醇A和B活性最强，IC50值分别为9.7 μM和9.6 μM。还评估了这些化合物与紫杉醇的协同作用，以及它们对细胞周期分布的影响和在克隆形成试验中的表现。两种化合物均与紫杉醇表现出显著的协同作用。