Larsen Nicolai B, Hickson Ian D, Mankouri Hocine W
a Center for Healthy Aging; Department of Cellular and Molecular Medicine ; University of Copenhagen ; Copenhagen , Denmark.
Cell Cycle. 2014;13(19):2994-8. doi: 10.4161/15384101.2014.958912.
The high-affinity binding of the Tus protein to specific 21-bp sequences, called Ter, causes site-specific, and polar, DNA replication fork arrest in E coli. The Tus-Ter complex serves to coordinate DNA replication with chromosome segregation in this organism. A number of recent and ongoing studies have demonstrated that Tus-Ter can be used as a heterologous tool to generate site-specific perturbation of DNA replication when reconstituted in eukaryotes. Here, we review these recent findings and explore the molecular mechanism by which Tus-Ter mediates replication fork (RF) arrest in the budding yeast, S. cerevisiae. We propose that Tus-Ter is a versatile, genetically tractable, and regulatable RF blocking system that can be utilized for disrupting DNA replication in a diverse range of host cells.
Tus蛋白与特定的21碱基对序列(称为Ter)的高亲和力结合会导致大肠杆菌中位点特异性且具有极性的DNA复制叉停滞。Tus-Ter复合物在该生物体中用于协调DNA复制与染色体分离。最近以及正在进行的一些研究表明,当在真核生物中重建时,Tus-Ter可作为一种异源工具来产生DNA复制的位点特异性扰动。在这里,我们回顾这些最新发现,并探讨Tus-Ter在芽殖酵母酿酒酵母中介导复制叉(RF)停滞的分子机制。我们提出,Tus-Ter是一种通用的、遗传上易于操作且可调控的RF阻断系统,可用于在多种宿主细胞中破坏DNA复制。