Menezes Bruna S, Solidade Lucas S, Conceição Aparecido A, Santos Junior Manoel N, Leal Patrícia L, de Brito Edy S, Canuto Kirley M, Mendonça Simone, de Siqueira Félix G, Marques Lucas M
Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia. Vitória da Conquista, Bahia, Brazil.
Embrapa Agroindústria Tropical, Fortaleza, Ceará, Brazil.
AMB Express. 2020 Jul 1;10(1):117. doi: 10.1186/s13568-020-01054-y.
The fungal kingdom has been widely studied as a source of bioactive compounds of interest to the pharmaceutical and food industry. This paper studies the production of natural red pigments by Fusarium solani BRM054066 in the submerged fermentation system, using Doehlert experimental design to determine optimal cultivation conditions. The chemical composition of the red pigment was determined by Nuclear Magnetic Resonance spectroscopy (NMR) and Ultra-Performance Liquid Chromatography coupled to Mass Spectrometry (UPLC-MS). Antioxidant activity was assessed by the ability to sequester of free radical DPPH. In the analysis of anti-inflammatory activity, murine peritoneal macrophages activated by LPS were used, and the gene expression of TNF-α, IL-1β, IL-6, IL-10 and IL-17 was determined using qPCR. As a result, it was found that agitation at 200 rpm and glucose concentration ≥ 20 g/L promote the best results in the production of red pigment. The chemical compounds identified were two naphthoquinones, fusarubin and dihydrofusarubin, and an anthraquinone, a bostrycoidin, being fusarubin the majority compound. The red pigment showed antioxidant activity by scavenge 50% of the DPPH radical, in a concentration of 24 µg/mL. The pigment also showed an effective anti-inflammatory capacity by reducing the overexpression of the pro-inflammatory cytokines TNF-α, IL-1β and IL-6 and promoting the production of anti-inflammatory IL-10 and IL-17, in murine macrophages activated by LPS (p < 0.05). According to the results, the fungus F. solani BRM054066, under optimized conditions of cultivation, proved to be a promising source of biologically active natural pigments with wide industrial applicability.
真菌界作为制药和食品工业感兴趣的生物活性化合物的来源已得到广泛研究。本文研究了茄病镰刀菌BRM054066在深层发酵系统中天然红色素的生产，采用Doehlert实验设计来确定最佳培养条件。通过核磁共振光谱（NMR）和超高效液相色谱-质谱联用（UPLC-MS）测定红色素的化学成分。通过清除自由基DPPH的能力评估抗氧化活性。在抗炎活性分析中，使用经脂多糖激活的小鼠腹腔巨噬细胞，并通过qPCR测定TNF-α、IL-1β、IL-6、IL-10和IL-17的基因表达。结果发现，200 rpm的搅拌速度和≥20 g/L的葡萄糖浓度能使红色素产量达到最佳效果。鉴定出的化合物有两种萘醌，即镰刀菌素和二氢镰刀菌素，以及一种蒽醌，即波斯特菌素，其中镰刀菌素是主要成分。红色素在浓度为24 μg/mL时通过清除50%的DPPH自由基显示出抗氧化活性。该色素还通过降低脂多糖激活的小鼠巨噬细胞中促炎细胞因子TNF-α、IL-1β和IL-6的过表达，并促进抗炎细胞因子IL-10和IL-17的产生，显示出有效的抗炎能力（p<0.05）。根据结果，茄病镰刀菌BRM054066在优化的培养条件下被证明是一种具有广阔工业应用前景的生物活性天然色素的有前途的来源。
