War Abdul Rashid, Paulraj Michael Gabriel, Ignacimuthu Savarimuthu, Sharma Hari Chand
International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Andhra Pradesh, India; Entomology Research Institute, Loyola College, Chennai, Tamil Nadu, India.
Pest Manag Sci. 2015 Jan;71(1):72-82. doi: 10.1002/ps.3764. Epub 2014 Apr 4.
Induced resistance to Helicoverpa armigera through exogenous application of jasmonic acid (JA) and salicylic acid (SA) was studied in groundnut genotypes (ICGV 86699, ICGV 86031, ICG 2271 and ICG 1697) with different levels of resistance to insects and the susceptible check JL 24 under greenhouse conditions. Activities of oxidative enzymes and the amounts of secondary metabolites and proteins were quantified at 6 days after JA and SA application/insect infestation. Data were also recorded on plant damage and H. armigera larval weights and survival.
Higher levels of enzymatic activities and amounts of secondary metabolites were observed in the insect-resistant genotypes pretreated with JA and then infested with H. armigera than in JL 24. The insect-resistant genotypes suffered lower insect damage and resulted in poor survival and lower weights of H. armigera larvae than JL 24. In some cases, JA and SA showed similar effects.
JA and SA induced the activity of antioxidative enzymes in groundnut plants against H. armigera, and reduced its growth and development. However, induced response to application of JA was greater than to SA, and resulted in reduced plant damage, and larval weights and survival, suggesting that induced resistance can be used as a component of pest management in groundnut.
在温室条件下，研究了通过外源施用茉莉酸（JA）和水杨酸（SA）对具有不同抗虫水平的花生基因型（ICGV 86699、ICGV 86031、ICG 2271和ICG 1697）以及感虫对照JL 24诱导对棉铃虫的抗性。在施用JA和SA/棉铃虫侵染后6天，对氧化酶活性、次生代谢物和蛋白质含量进行了定量分析。还记录了植株损伤情况、棉铃虫幼虫体重和存活率的数据。
与JL 24相比，先用JA预处理然后接种棉铃虫的抗虫基因型中，酶活性水平和次生代谢物含量更高。抗虫基因型遭受的虫害较轻，棉铃虫幼虫的存活率较低，体重也比JL 24轻。在某些情况下，JA和SA表现出相似的效果。
JA和SA诱导花生植株中抗氧化酶的活性以抵御棉铃虫，并降低其生长和发育。然而，对JA施用的诱导反应大于对SA的反应，从而减少了植株损伤、幼虫体重和存活率，这表明诱导抗性可作为花生害虫管理的一个组成部分。
