Ahmad Sohail, Ahmad Shabir, Bibi Ahtaram, Ishaq Muhammad Saqib, Afridi Muhammad Siddique, Kanwal Farina, Zakir Muhammad, Fatima Farid
Department of Chemistry, Kohat University of Science and Technology, Kohat 26000, Pakistan.
Department of Chemistry, Islamia College University, Peshawar 25120, Pakistan.
ScientificWorldJournal. 2014;2014:829076. doi: 10.1155/2014/829076. Epub 2014 Nov 12.
Heliotropium bacciferum is paramount in medicinal perspective and belongs to Boraginaceae family. The crude and numerous fractions of leaves, stem, and roots of the plant were investigated for phytochemical analysis and DPPH radical scavenging activity. Phytochemical analysis of crude and fractions of the plant revealed the presence of alkaloids, saponins, tannins, steroids, terpenoids, flavonoids, glycosides, and phenols. The antioxidant (free radical scavenging) activity of various extracts of the Heliotropium bacciferum was resolute against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical with the avail of UV spectrophotometer at 517 nm. The stock solution (1000 mg/mL) and then several dilutions (50, 100, 150, 200, and 250 mg/mL) of the crude and fractions were prepared. Ascorbic acid was used as a standard. The plant leaves (52.59 ± 0.84 to 90.74 ± 1.00), stem (50.19 ± 0.92 to 89.42 ± 1.10), and roots extracts (49.19 ± 0.52 to 90.01 ± 1.02) divulged magnificent antioxidant activities. For the ascertainment of the fatty acid constituents a gas chromatograph hyphenated to mass spectrometer was used. The essential fatty acids for growth maintenance such as linoleic acid (65.70%), eicosadienoic acid (15.12%), oleic acid (8.72%), and palmitic acid (8.14%) were found in high percentage. The infrared spectra of all extracts of the plant were recorded by IR Prestige-21 FTIR model.
浆果状天芥菜在医学方面至关重要，属于紫草科。对该植物的叶、茎和根的粗提物及多种提取物进行了植物化学分析和DPPH自由基清除活性研究。对该植物粗提物和提取物的植物化学分析表明，其中存在生物碱、皂苷、单宁、甾体、萜类、黄酮类、糖苷和酚类。利用紫外可见分光光度计在517nm波长下，测定了浆果状天芥菜各种提取物对2,2-二苯基-1-苦基肼（DPPH）自由基的抗氧化（自由基清除）活性。制备了粗提物和提取物的储备溶液（1000mg/mL），然后进行了几种稀释（50、100、150、200和250mg/mL）。以抗坏血酸作为标准品。该植物的叶提取物（52.59±0.84至90.74±1.00）、茎提取物（50.19±0.92至89.42±1.10）和根提取物（49.19±0.52至90.01±1.02）均表现出显著的抗氧化活性。使用气相色谱-质谱联用仪测定脂肪酸成分。发现对维持生长至关重要的必需脂肪酸，如亚油酸（65.70%）、二十碳二烯酸（15.12%）、油酸（8.72%）和棕榈酸（8.14%）的含量很高。通过IR Prestige-21傅里叶变换红外光谱仪记录了该植物所有提取物 的红外光谱。
