Thibeault Susan L, Rousseau Bernard, Welham Nathan V, Hirano Shigeru, Bless Diane M
Division of Otolaryngology-Head and Neck Surgery, The University of Utah School of Medicine, Salt Lake City, Utah, USA.
Laryngoscope. 2004 Apr;114(4):760-4. doi: 10.1097/00005537-200404000-00031.
OBJECTIVES/HYPOTHESIS: The objective was to measure the level of hyaluronan during the first 15 days after vocal fold biopsy in a rabbit model.
Experimental, nonrandomized prospective study.
Twenty-eight rabbits underwent unilateral vocal fold biopsy. The contralateral vocal fold was preserved as a control sample. On days 3, 5, 10, and 15 after biopsy, hyaluronan levels in the injured and normal vocal folds were measured immunohistologically and with an ELISA assay.
Hyaluronan levels in the injured vocal fold were lowest on day 3 and highest on day 5 after injury. Statistical analysis revealed that the injured vocal fold demonstrated significantly lower levels of hyaluronan on day 3 than on days 5, 10, and 15. On day 5, the injured vocal fold demonstrated significantly greater levels of hyaluronan than those observed on days 3, 10, and 15. Compared with the normal vocal fold, the injured vocal fold demonstrated significantly lower levels of hyaluronan on days 3, 10, and 15. No differences were observed between the injured and normal vocal fold on day 5, when the hyaluronan level was maximized in the injured vocal fold.
Maximizing hyaluronan levels in the early stages of wound repair may have therapeutic potential for decreasing the incidence of vocal fold scar. Decreased hyaluronan levels may provide a less than optimal environment for normal tissue regeneration and may contribute to the formation of scar tissue in vocal fold lamina propria.
目的/假设：目的是在兔模型中测量声带活检后前15天内透明质酸的水平。
实验性、非随机前瞻性研究。
28只兔子接受单侧声带活检。对侧声带作为对照样本保留。在活检后第3、5、10和15天，通过免疫组织化学和酶联免疫吸附测定法测量受伤和正常声带中的透明质酸水平。
受伤声带中的透明质酸水平在损伤后第3天最低，第5天最高。统计分析显示，受伤声带在第3天的透明质酸水平显著低于第5、10和15天。在第5天，受伤声带的透明质酸水平显著高于第3、10和15天。与正常声带相比，受伤声带在第3、10和15天的透明质酸水平显著降低。在第5天，受伤声带的透明质酸水平达到最大值，此时受伤声带与正常声带之间未观察到差异。
在伤口修复早期使透明质酸水平最大化可能具有降低声带瘢痕发生率的治疗潜力。透明质酸水平降低可能为正常组织再生提供不太理想的环境，并可能导致声带固有层瘢痕组织的形成。
