sp. nov. Isolated from Soil with Potential Antioxidant Activity.

A white-pigmented, non-motile, gram-negative, and rod-shaped bacterium, designated CYS-02, was isolated from soil sampled at Suwon, Gyeonggi-do, Republic of Korea. Cells were strictly aerobic, grew optimally at 20-28ºC and hydrolyzed Tween 40. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CYS-02 formed a lineage within the family and clustered as members of the genus . The closest members were DSM 27352 (98.6% sequence similarity), NBRC 15149 (98.5%), and JM-310 (98.3%). The principal respiratory quinone was Q-8 and the major polar lipids contain phosphatidylethanolamine (PE), phosphatidylethanolamine (PG), and diphosphatidylglycerol (DPG). The predominant cellular fatty acids were C, summed feature 3 (C7c and/or C6c) and summed feature 8 (C7c and/or C6c). The DNA GC content was 67.7 mol%. The ANI and dDDH values between strain CYS-02 and the closest members in the genus were ≤ 79.0 and 22.4%, respectively, and the AAI and POCP values between CYS-02 and the other related species in the family were > 70% and > 50%, respectively. The genome of strain CYS-02 showed a putative terpene biosynthetic cluster responsible for antioxidant activity which was supported by DPPH radical scavenging activity test. Based on genomic, phenotypic and chemotaxonomic analyses, strain CYS-02 was classified into a novel species in the genus , for which the name sp. nov., has been proposed. The type strain is CYS-02 (= KACC 22656 = NBRC 115645 [corrected] ).