Thammina Chandra S, Olsen Richard T, Malapi-Wight Martha, Crouch Jo Anne, Pooler Margaret R
United States Department of Agriculture, Agricultural Research Service (USDA-ARS), U.S. National Arboretum, Floral and Nursery Plants Research Unit, 10300 Baltimore Avenue, Building 010A, Beltsville, Maryland 20705 USA ; Department of Plant Biology and Pathology, Rutgers University, 59 Dudley Road, New Brunswick, New Jersey 08901 USA.
United States Department of Agriculture, Agricultural Research Service (USDA-ARS), U.S. National Arboretum, Floral and Nursery Plants Research Unit, 10300 Baltimore Avenue, Building 010A, Beltsville, Maryland 20705 USA.
Appl Plant Sci. 2014 Nov 28;2(12). doi: 10.3732/apps.1400095. eCollection 2014 Dec.
Genic microsatellites or simple sequence repeat (genic-SSR) markers were developed in boxwood (Buxus taxa) for genetic diversity analysis, identification of taxa, and to facilitate breeding. •
cDNA libraries were developed from mRNA extracted from leaves of Buxus sempervirens 'Vardar Valley' and sequenced using the Illumina MiSeq system. Approximately 11.9 million base pairs of sequence data were examined and 845 genic-SSRs were identified, including 469 dinucleotide, 360 trinucleotide, seven tetranucleotide, one pentanucleotide, and eight hexanucleotide repeats. Primer pairs were designed for 71 selectively chosen genic-SSRs containing trinucleotide repeat motifs and were used to amplify the corresponding loci in 18 diverse boxwood accessions. Twenty-three primer pairs amplified polymorphic loci, with two to 10 alleles per locus. •
These novel polymorphic genic-SSR markers will aid in evaluating genetic diversity of boxwood germplasm and allow verification of hybrids and cultivars for breeding programs.
在黄杨属植物中开发基因微卫星或简单序列重复(genic-SSR)标记,用于遗传多样性分析、分类群鉴定及辅助育种。
从欧洲黄杨‘瓦尔达尔谷’叶片提取的mRNA构建cDNA文库,并使用Illumina MiSeq系统进行测序。检查了约1190万个碱基对的序列数据,鉴定出845个genic-SSR,包括469个二核苷酸、360个三核苷酸、7个四核苷酸、1个五核苷酸和8个六核苷酸重复序列。为71个选择性选择的含有三核苷酸重复基序的genic-SSR设计引物对,并用于扩增18个不同黄杨属材料中的相应位点。23对引物扩增出多态性位点,每个位点有2至10个等位基因。
这些新型多态性genic-SSR标记将有助于评估黄杨属种质的遗传多样性,并可用于育种计划中杂种和品种的鉴定。