Yamani Hanaa, Mantri Nitin, Morrison Paul D, Pang Edwin
School of Applied Sciences, Health Innovations Research Institute, RMIT University, Melbourne 3000, Victoria, Australia.
BMC Complement Altern Med. 2014 Dec 15;14:495. doi: 10.1186/1472-6882-14-495.
The foraging choices of honey bees are influenced by many factors, such as floral aroma. The composition of volatile compounds influences the bioactivity of the aromatic plants and honey produced from them. In this study, Agastache rugosa was evaluated as part of a project to select the most promising medicinal plant species for production of bioactive honey.
Headspace solid-phase microextraction HS-SPME /GC-MS was optimized to identify the volatile bioactive compounds in the leaves, flower spikes, and for the first time, the flower nectar of Australian grown A. rugosa.
Methyl chavicol (= estragole) was the predominant headspace volatile compound in the flowers with nectar, flower spikes, and leaves, with a total of 97.16%, 96.74% and 94.35%, respectively. Current results indicate that HS-SPME/GC-MS could be a useful tool for screening estragole concentration in herbal products.
Recently, estragole was suspected to be carcinogenic and genotoxic, according to the European Union Committee on Herbal Medicinal Products. Further studies are needed on safe daily intake of Agastache as herbal tea or honey, as well as for topical uses.
蜜蜂的觅食选择受多种因素影响，如花香。挥发性化合物的组成会影响芳香植物及其所产蜂蜜的生物活性。在本研究中，作为选择最具潜力的药用植物以生产具有生物活性蜂蜜项目的一部分，对藿香进行了评估。
优化了顶空固相微萃取（HS-SPME）/气相色谱-质谱联用（GC-MS）技术，以鉴定澳大利亚种植的藿香的叶片、花穗以及首次对花蜜中的挥发性生物活性化合物进行分析。
甲基丁香酚（= 草蒿脑）是含花蜜的花朵、花穗和叶片中主要的顶空挥发性化合物，分别占总量的97.16%、96.74%和94.35%。目前的结果表明，HS-SPME/GC-MS可能是筛选草药产品中草蒿脑浓度的有用工具。
根据欧盟草药产品委员会的规定，最近草蒿脑被怀疑具有致癌性和基因毒性。需要进一步研究将藿香作为草药茶或蜂蜜的安全日摄入量，以及其局部使用情况。
