El-Domyati Moetaz, El-Ammawi Tarek S, Medhat Walid, Moawad Osama, Mahoney Mỹ G, Uitto Jouni
Department of Dermatology, Al-Minya University, Al-Minya, Egypt.
Int J Dermatol. 2015 Apr;54(4):396-404. doi: 10.1111/ijd.12435. Epub 2014 Dec 16.
Transforming growth factor-β (TGF-β) is a major regulator of the synthesis of extracellular matrix (ECM) proteins in human skin as it stimulates fibroblast proliferation and collagen production. Perturbed TGF-β expression may play a key role in the pathogenesis of skin aging.
This study was conducted to objectively evaluate the effects of different modalities of non-invasive facial rejuvenation on TGF-β expression and to correlate its level with that of newly synthesized collagen.
A total of 36 patients with Fitzpatrick skin types III and IV were divided into six groups. Each group of six patients was subjected to a different non-invasive modality for the treatment of skin aging, including radiofrequency (RF), Nd:YAG 1320-nm laser and Er:YAG 2940-nm laser mini-peels, intense pulsed light (IPL), mesotherapy injection, and electro-optical synergy (ELOS). Skin biopsies were obtained before treatment, at the end of treatment, and at three months post-treatment. In addition, biopsies were obtained from 30 control subjects. Levels of TGF-β were quantitatively evaluated using computerized image analysis of immunostained sections.
The expression of TGF-β was statistically significantly increased (P < 0.05) at the end of Nd:YAG 1320-nm and Er:YAG 2940-nm mini-peel treatments compared with baseline levels, and at three months post-treatment with RF and ELOS compared with pretreatment and end-of-treatment levels. However, no significant differences (P > 0.05) were observed in TGF-β level in response to IPL or mesotherapy treatments in comparison with baseline. The level of TGF-β was positively correlated (P < 0.05) to that of newly synthesized collagen at the end of Nd:YAG 1320-nm laser and Er:YAG 2940-nm laser mini-peels, as well as at three months after RF and ELOS treatments.
Radiofrequency, ELOS, and Nd:YAG 1320-nm laser and Er:YAG 2940-nm laser mini-peels resulted in an increase in TGF-β expression, which may mediate the effects of these modalities in enhancing dermal collagen expression through the activation of fibroblasts and thereby reverse the photoaging of skin.
转化生长因子-β（TGF-β）是人类皮肤细胞外基质（ECM）蛋白合成的主要调节因子，因为它能刺激成纤维细胞增殖和胶原蛋白生成。TGF-β表达紊乱可能在皮肤衰老的发病机制中起关键作用。
本研究旨在客观评估不同非侵入性面部年轻化方式对TGF-β表达的影响，并将其水平与新合成胶原蛋白的水平相关联。
总共36例Fitzpatrick皮肤分型为III型和IV型的患者被分为六组。每组6例患者接受不同的非侵入性皮肤衰老治疗方式，包括射频（RF）、钕：钇铝石榴石1320纳米激光和铒：钇铝石榴石2940纳米激光微剥脱、强脉冲光（IPL）、中胚层疗法注射和光电协同（ELOS）。在治疗前、治疗结束时和治疗后三个月获取皮肤活检样本。此外，从30名对照受试者获取活检样本。使用免疫染色切片的计算机图像分析对TGF-β水平进行定量评估。
与基线水平相比，钕：钇铝石榴石1320纳米和铒：钇铝石榴石2940纳米微剥脱治疗结束时，TGF-β表达在统计学上显著增加（P < 0.05）；与治疗前和治疗结束时水平相比，射频和ELOS治疗后三个月时TGF-β表达也显著增加。然而，与基线相比，IPL或中胚层疗法治疗后TGF-β水平未观察到显著差异（P > 0.05）。在钕：钇铝石榴石1320纳米激光和铒：钇铝石榴石2940纳米激光微剥脱结束时，以及射频和ELOS治疗后三个月时，TGF-β水平与新合成胶原蛋白水平呈正相关（P < 0.05）。
射频、ELOS以及钕：钇铝石榴石1320纳米激光和铒：钇铝石榴石2940纳米激光微剥脱导致TGF-β表达增加，这可能介导这些治疗方式通过激活成纤维细胞增强真皮胶原蛋白表达的作用，从而逆转皮肤光老化。
