Widowati Wahyu, Faried Ahmad, Adam Achmad, Rahmat Deni, Kusuma Hanna Sari Widya, Dewi Nindia Salsabila Mia, Gondokesumo Marisca Evalina, Rizal Rizal, Nainggolan Ita Margaretha, Vosough Massoud
Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia.
Department of Neurosurgery, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung, West Java, Indonesia.
Iran J Basic Med Sci. 2024;27(7):868-878. doi: 10.22038/IJBMS.2024.70825.15385.
Skin aging is a degenerative process that can be induced by UV irradiation. UV radiation can produce reactive oxidate stress which causes premature aging. This study aims to examine the antiaging potential of secretome gel (SC) from human Wharton Jelly Mesenchymal Stem Cells (hWJ-MSCs) in a UVB-induced mice model.
The secretome was obtained from hWJ-MSCs and made in gel form. Male mice were radiated by UVB for 15 min twice daily for 14 days. The gel was topically applied to the mice's dorsal skin. Two treatments of secretome gel: secretome 1 is applied once and secretome 2 is applied twice daily after UVB radiation. TGF-β1, IL-10, and IL-18 gene expression was determined using RT-PCR. Hematoxylin Eosin staining was used to observe the inflammation and collagen density of skin tissue. An immunohistochemistry assay was used to analyze the protein expression of P53, COL4A1, MMP-2, and MMP-13. The data were statistically analyzed using the ANOVA test followed by the Tukey post hoc test (<0.05).
UVB induction caused loss of collagen, increasing inflammation and high expression of aging mediators. SC increased the gene expression of TGF-β1 and IL-10 and decreased IL-18 gene expression. Histopathological tests showed that SG increased collagen density, lowered inflammation, and repaired cell damage in skin tissue. Immunohistochemistry test showed that SC decreased MMP-2, MMP-13, and P53 expression, in contrast, increased COL4A1.
The secretome gel of hWJ-MSCs showed antiaging activities with potential for preventing and curing skin aging.
皮肤老化是一种可由紫外线照射诱导的退行性过程。紫外线辐射可产生活性氧化应激，从而导致早衰。本研究旨在检测人脐带华通氏胶间充质干细胞（hWJ-MSCs）分泌组凝胶（SC）在紫外线B诱导的小鼠模型中的抗老化潜力。
从hWJ-MSCs中获取分泌组并制成凝胶形式。雄性小鼠每天接受两次紫外线B照射，每次15分钟，共照射14天。将凝胶局部涂抹于小鼠背部皮肤。分泌组凝胶有两种处理方式：分泌组1在紫外线B照射后涂抹一次，分泌组2在紫外线B照射后每天涂抹两次。使用逆转录聚合酶链反应（RT-PCR）测定转化生长因子-β1（TGF-β1）、白细胞介素-10（IL-10）和白细胞介素-18（IL-18）基因表达。采用苏木精-伊红染色观察皮肤组织的炎症和胶原密度。采用免疫组织化学分析检测P53、IV型胶原α1链（COL4A1）、基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-13（MMP-13）的蛋白表达。数据采用方差分析（ANOVA）检验，随后进行Tukey事后检验（<0.05）进行统计学分析。
紫外线B诱导导致胶原蛋白流失、炎症增加以及衰老介质的高表达。SC增加了TGF-β1和IL-10的基因表达，并降低了IL-18基因表达。组织病理学检测表明，SC增加了皮肤组织中的胶原密度，减轻了炎症，并修复了细胞损伤。免疫组织化学检测表明，SC降低了MMP-2、MMP-13和P53的表达，相反，增加了COL4A1的表达。
hWJ-MSCs分泌组凝胶具有抗老化活性，具有预防和治疗皮肤老化的潜力。
