Diban N, Ramos-Vivas J, Remuzgo-Martinez S, Ortiz I, Urtiaga A
Department of Chemical and Biomolecular Engineering. University of Cantabria. Av. de los Castros s/n. 39005 Santander, Spain.
Curr Top Med Chem. 2014;14(23):2743-9. doi: 10.2174/1568026614666141215153932.
The regeneration of brain tissue is one of the major challenges in regenerative medicine due to the lack of viable grafts to support the re-growth of functional tissue after a traumatic injury. The development of biocompatible and biodegradable structures with appropriate morphology for the interaction with neural tissue is required. The objective pursued in this work is to develop a biodegradable 2D scaffold structure for neural tissue engineering. Poly(ε-caprolactone) (PCL) was the selected material due to its biocompatibility and biodegradability in the long term. PCL (15%w/w) was dissolved in N-methylpyrrolidone and the film was fabricated by phase inversion casting technique employing ethanol and isopropanol as coagulation baths. The physical structure, morphology and topography of the flat scaffolds were characterized using different techniques. The two different scaffolds presented homogeneous structure with high porosity (higher than 85%), contact angles higher than 90(o), high roughness (Ra> 0.6 μm) and superficial pore sizes of 0.7 and 1.7 μm, respectively. Permeance tests showed high water permeabilities (~350-590 mL m(-1) bar(-1) h(-1)) indicative of promising nutrients supply to the cells. Finally, in vitro human glioblastoma cells cultures after 48 hours showed good cell attachment, proliferation and penetration in the scaffolds. Detailed evaluation of the interaction between the surface morphology and the properties of the scaffolds with the cell response has been done. Thus, the PCL films herein fabricated show promising results as scaffolds for neural tissue regeneration.
由于缺乏可行的移植物来支持创伤性损伤后功能组织的再生，脑组织再生是再生医学面临的主要挑战之一。需要开发具有适当形态以与神经组织相互作用的生物相容性和可生物降解结构。这项工作的目标是开发一种用于神经组织工程的可生物降解二维支架结构。聚（ε-己内酯）（PCL）因其生物相容性和长期可生物降解性而被选为材料。将PCL（15%w/w）溶解在N-甲基吡咯烷酮中，并采用乙醇和异丙醇作为凝固浴，通过相转化浇铸技术制备薄膜。使用不同技术对扁平支架的物理结构、形态和表面形貌进行了表征。两种不同的支架呈现出均匀的结构，孔隙率高（高于85%），接触角大于90°，粗糙度高（Ra>0.6μm），表面孔径分别为0.7μm和1.7μm。渗透测试显示出高透水性（约350-590 mL m(-1) bar(-1) h(-1)），表明有望为细胞提供营养物质。最后，48小时后的体外人胶质母细胞瘤细胞培养显示细胞在支架中具有良好的附着、增殖和渗透能力。已对表面形态与支架性能之间的相互作用以及细胞反应进行了详细评估。因此，本文制备的PCL薄膜作为神经组织再生支架显示出有前景的结果。
