Pepose J S
Department of Ophthalmology, Washington University School of Medicine, St Louis, MO 63110.
Curr Eye Res. 1989 Aug;8(8):851-8. doi: 10.3109/02713688909000875.
The corneal migration and topographic distribution of Langerhans cells (LC) in relation to herpes simplex virus antigens was studied during the course of dendritic keratitis in inbred mice. Corneal epithelial sheets from infected mice at selected time points were "double stained" for Ia-positive Langerhans cells and HSV antigens, using a sequential avidin biotin immunoperoxidase and glucose oxidase technique. The amount of HSV antigen was maximum at day 2 paralleling the clinical time course, with most corneal epithelium HSV antigen negative by day 8. LC were seen in peripheral corneas by day 2 and in paracentral and central cornea by day 8, with peak numbers detected between days 8 and 11 post-infection. Although HSV antigens and LC were simultaneously detected within corneal epithelium, LC were not observed in anatomic juxtaposition to HSV antigens, even after reinoculation of infected corneas with HSV on day 14 following the primary infection. These data suggest that local factors in the corneal epithelium other than HSV antigens per se may be chemotactic for LC during the course of dendritic keratitis.
在近交系小鼠的树枝状角膜炎病程中，研究了朗格汉斯细胞（LC）与单纯疱疹病毒抗原相关的角膜迁移和地形图分布。使用顺序抗生物素蛋白生物素免疫过氧化物酶和葡萄糖氧化酶技术，对选定时间点感染小鼠的角膜上皮片进行Ia阳性朗格汉斯细胞和HSV抗原的“双重染色”。HSV抗原量在第2天达到最大值，与临床病程平行，到第8天大多数角膜上皮HSV抗原呈阴性。感染后第2天在周边角膜可见LC，第8天在旁中心和中央角膜可见LC，在感染后第8至11天检测到LC数量峰值。尽管在角膜上皮内同时检测到HSV抗原和LC，但即使在初次感染后第14天用HSV重新接种感染的角膜，也未观察到LC与HSV抗原在解剖位置上并列。这些数据表明，在树枝状角膜炎病程中，除HSV抗原本身外，角膜上皮中的局部因素可能对LC具有趋化作用。
