Navarro Marta, Fiore Alberto, Fogliano Vincenzo, Morales Francisco J
Institute of Food Science, Technology and Nutrition, ICTAN-CSIC, E-28040 Madrid, Spain.
Food Funct. 2015 Feb;6(2):574-83. doi: 10.1039/c4fo01049c.
The use of natural compounds as antiglycative agents to reduce the load of advanced glycation end products from diet is very promising. Olive mill wastewater is a by-product of the olive oil extraction processes with a high content of hydroxytyrosol, hydroxytyrosol derivatives and molecules containing o-dihydroxyl functions such as verbascoside. Two powders were obtained after the ultrafiltration and nanofiltration of olive mill wastewater, and successive spray drying with maltodextrin and acacia fiber. The samples were characterized by phenolic composition and antioxidant capacity. Antiglycative capacity was evaluated by in vitro BSA-glucose and BSA-methylglyoxal assays, formation of Amadori products and direct trapping of reactive dicarbonyls (methylglyoxal and glyoxal). Both ultrafiltered and nanofiltered olive mill wastewater powders had an activity comparable to quercetin and hydroxytyrosol against the inhibition of protein glycation (IC50 = 0.3 mg mL(-1)). The antiglycative activity of the powder was further investigated after separation by reverse phase solid extraction. Fractions extracted with the methanol content higher than 40% and rich in hydroxytyrosol and verbascoside exerted the highest reactivity against dicarbonyls. Data confirmed that the direct trapping of dicarbonyl compounds is the main route explaining the antiglycative action rather than of the already known antioxidant capacity. Results support further investigations to evaluate the technological feasibility to use olive mill wastewater powders as antiglycative ingredients in foods or in pharmacological preparations in future.
使用天然化合物作为抗糖化剂以减少饮食中晚期糖基化终产物的负荷是非常有前景的。橄榄油厂废水是橄榄油提取过程的副产品，含有高含量的羟基酪醇、羟基酪醇衍生物以及含有邻二羟基官能团的分子，如毛蕊花糖苷。经过对橄榄油厂废水进行超滤和纳滤，然后与麦芽糊精和阿拉伯胶纤维连续喷雾干燥后，得到了两种粉末。通过酚类成分和抗氧化能力对样品进行了表征。通过体外牛血清白蛋白 - 葡萄糖和牛血清白蛋白 - 甲基乙二醛测定、阿马多里产物的形成以及活性二羰基化合物（甲基乙二醛和乙二醛）的直接捕获来评估抗糖化能力。超滤和纳滤的橄榄油厂废水粉末对蛋白质糖基化的抑制活性与槲皮素和羟基酪醇相当（IC50 = 0.3 mg mL(-1)）。通过反相固相萃取分离后，进一步研究了该粉末的抗糖化活性。用甲醇含量高于40%且富含羟基酪醇和毛蕊花糖苷的馏分对二羰基化合物具有最高的反应活性。数据证实，二羰基化合物的直接捕获是解释抗糖化作用的主要途径，而不是已知的抗氧化能力。结果支持进一步研究，以评估未来将橄榄油厂废水粉末用作食品或药物制剂中抗糖化成分的技术可行性。
