Cornelissen M T, van den Tweel J G, Struyk A P, Jebbink M F, Briët M, van der Noordaa J, ter Schegget J T
Department of Medical Microbiology, University of Amsterdam, The Netherlands.
J Gen Virol. 1989 Oct;70 ( Pt 10):2555-62. doi: 10.1099/0022-1317-70-10-2555.
The localization of human papillomavirus type 16 (HPV-16) DNA throughout the cervix uteri of women with cervical intraepithelial neoplasia (CIN) was studied by utilizing the polymerase chain reaction technique directly on histologically defined sections of paraffin-embedded cervical tissue obtained by conizations. HPV-16 DNA was detected only in the sections that contained CIN lesions and/or koilocytes. No HPV-16 DNA was detected in sections that contained only normal epithelium. This is in accordance with HPV-16 playing a role in the development of CIN lesions.
利用聚合酶链反应技术，直接对通过锥切术获得的石蜡包埋宫颈组织的组织学定义切片进行研究，以确定16型人乳头瘤病毒（HPV-16）DNA在宫颈上皮内瘤变（CIN）女性整个子宫颈中的定位。仅在含有CIN病变和/或空泡细胞的切片中检测到HPV-16 DNA。在仅含有正常上皮的切片中未检测到HPV-16 DNA。这与HPV-16在CIN病变发展中起作用是一致的。
