Regiel-Futyra Anna, Kus-Liśkiewicz Małgorzata, Sebastian Victor, Irusta Silvia, Arruebo Manuel, Stochel Grażyna, Kyzioł Agnieszka
Faculty of Chemistry, Jagiellonian University , Ingardena 3, 30-060 Kraków, Poland.
ACS Appl Mater Interfaces. 2015 Jan 21;7(2):1087-99. doi: 10.1021/am508094e. Epub 2015 Jan 8.
This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity. The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it. Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration. Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials. Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa. The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells. They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells. This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.
这项工作描述了具有高抗菌活性的非细胞毒性纳米复合材料（胶体或薄膜形式）的合成与表征。生物相容性和可生物降解的聚合物壳聚糖被用作还原剂和稳定剂，用于合成嵌入其中的金纳米颗粒。在此，首次使用了三种平均分子量和脱乙酰度（DD）不同的壳聚糖等级，并优化了金前驱体浓度。为了获得具有抗菌性但无细胞毒性的纳米复合材料，对几个因素进行了分析。基于中等分子量和最高DD的壳聚糖薄膜对金黄色葡萄球菌和铜绿假单胞菌的生物膜形成菌株表现出最高的抗菌活性。所得纳米复合材料对哺乳动物体细胞和肿瘤细胞未显示任何细胞毒性。它们对细菌细胞壁产生破坏作用，而在真核细胞上其内化受到阻碍。这种选择性和安全性使其有可能作为抗菌涂层应用于生物医学领域。
