College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Key Laboratory of Biogeography and Bioresources in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Xinjiang 830011, PR China.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4064-4071. doi: 10.1099/ijs.0.050096-0. Epub 2013 May 31.
A strictly aerobic, Gram-stain-positive, motile and spore-forming bacterium, strain 3nP4(T), was isolated from the Puge hot spring located in the south-western geothermal area of China. Strain 3nP4(T) grew at 38-66 °C (optimum 57-60 °C), at pH 6.0-9.3 (optimum 7.0-7.5) and with 0-4 % (w/v) NaCl (optimum 0-0.5 %). Phylogenetic analysis of 16S rRNA gene sequences, as well as DNA-DNA relatedness values, indicated that the isolate represents a novel species of the genus Anoxybacillus, related most closely to Anoxybacillus voinovskiensis DSM 12111(T). Strain 3nP4(T) had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and one unidentified phospholipid as major polar lipids and iso-C15 : 0 and iso-C17 : 0 as major fatty acids, which are both typical chemotaxonomic characteristics of the genus Anoxybacillus. The mean DNA G+C content of strain 3nP4(T) was 39.2±0.95 mol% (HPLC). A distinctive characteristic of the novel isolate was its extreme reliance on vitamin mixture or yeast extract for growth. Based on data from this taxonomic study using a polyphasic approach, strain 3nP4(T) is considered to represent a novel species of the genus Anoxybacillus, for which the name Anoxybacillus vitaminiphilus sp. nov. is proposed. The type strain is 3nP4(T) ( = CGMCC 1.8979(T) = JCM 16594(T)).
一株严格需氧、革兰氏阳性、运动和产芽孢的细菌，菌株 3nP4(T)，从位于中国西南地热区的普格温泉中分离得到。菌株 3nP4(T)在 38-66°C（最佳温度 57-60°C）、pH6.0-9.3（最佳 pH7.0-7.5）和 0-4%（w/v）NaCl（最佳 0-0.5%）的条件下生长。16S rRNA 基因序列的系统发育分析以及 DNA-DNA 同源性值表明，该分离株代表了一个新型的厌氧芽孢杆菌属，与厌氧芽孢杆菌 voinovskiensis DSM 12111(T)最为密切相关。菌株 3nP4(T)的主要极性脂为二磷酸甘油酯、磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的磷脂，主要脂肪酸为 iso-C15：0 和 iso-C17：0，这都是厌氧芽孢杆菌属的典型化学分类特征。菌株 3nP4(T)的平均 DNA G+C 含量为 39.2±0.95 mol%（HPLC）。该新型分离株的一个显著特征是其对维生素混合物或酵母提取物的生长极度依赖。基于多相分类研究的数据，菌株 3nP4(T)被认为代表了厌氧芽孢杆菌属的一个新种，现提议将其命名为维生素依赖厌氧芽孢杆菌（Anoxybacillus vitaminiphilus）。其模式菌株为 3nP4(T)（=CGMCC 1.8979(T)=JCM 16594(T)）。
